TSKgel SuperQ-5PW Anion Exchange Media
TSKgel SuperQ-5PW is especially suited for industrial purification of oligonucleotides
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The resin of choice for large scale oligonucleotide purification.
Oligonucleotide purification
TSK Gel SuperQ 5PW (20) displays excellent binding capacity and performance, is easy to use, robust (low back pressure and chemically resistant) and offers reproducible results over a long time span. High FLP-recovery and favourable peak shape.
Review Date: 15 May 2024 | Tosoh Bioscience - Separations
The TSKgel SuperQ-5PW resin is a strong anion exchange resin for biomolecule purification which is especially suited to purify oligos from longmers and shortmers. It is composed of highly crosslinked polymethacrylate beads that have been functionalized with quaternary amine (Q) strong anion exchange groups. TSKgel SuperQ-5PW is available in two particle sizes: 30 µm and 20 µm.
TSKgel SuperQ-5PW resins are best used for high resolution process polishing steps and for the purification of therapeutic oligonucleotides from synthesis impurities. It is applied in industrial manufacturing processes of approved siRNA therapeutics.
TSKgel Super-Q Features:
- High binding capacity for nucleic acids and proteins
- Efficient N-X and N+1 impurity removal at high oligo loadings
- High mechanical and chemical stability
Development of a universal preparative AEX method to purify oligonucleotides
One of the most widely used modifications of synthetic oligonucleotides is the phosphorothioate modification of the oligonucleotide backbone, a process which can lead to impurities. Tosoh Bioscience presents a universal method for the purification of phosphorothioate oligonucleotides using TSKgel® SuperQ-5PW (20), a specially designed resin which provides high resolution and selectivity. This method is presented as a starting point in the lab to develop efficient large-scale preparative processes. Explore Tosoh Bioscience's recommended method for purifying oligonucleotides on TSKgel SuperQ-5PW (20): at elevated temperature, using a 10 mmol/L sodium hydroxide buffer at pH 12 and sodium chloride as eluent.
(U)HPLC analysis of biomolecules
This compendium, from Tosoh Bioscience, compiles more than twenty application notes covering important aspects of biopharmaceutical analysis such as protein aggregation, charge isoforms and glycosylation as well as determination of drug-to-antibody ratio of antibody-drug-conjugates. The last section of the notebook provides tips and tricks to improve chromatographic separation of biomolecules.
