True MicroChIP Kit
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This microChIP-seq assay has been validated with the new generation IP-Star® Compact Automated Workstation. Furthermore Diagenode's high quality ChIP-seq grade antibodies have been used. Features: Revolutionary: Only 10.000 cells needed for complete ChIP-seq procedure Relia…
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We are using this kit to prepare primary samples for ChIPseq
Some of the instructions were a little difficult to understand, and the sonication optimization might be challenging if one has not performed ChIP before , but overall the kit is very easy and fast to use. At least for ChIP qPCR for certain histone marks, I would definitely recommend the microChIP kit.
Review Date: 23 Mar 2015 | Diagenode
for ChIP and ChIP-seq
This kit works very well for performing ChIP with small starting numbers of cells/amount of chromatin; in terms of qPCR, I get comparable Ct values when using 50ug chromatin with the microChIP kit vs. 200ug with a typical ChIP protocol, which is great when working with limited starting material (primary human samples). Starting with 50ug of chromatin from human samples (and using good antibodies), I was able to retain enough DNA to do library preparation using a "traditional" Illumina kit (i.e., not optimized for small amounts of starting material). When used with mouse cells, I get reproducible results when starting with only 5ug of chromatin. The only complaint I have is that an additional reagent (namely HBSS) is needed for the protocol, and this isn't clearly stated in the product information - I didn't notice it until I was setting up the reactions.
Review Date: 27 Feb 2015 | Diagenode
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells.
This microChIP-seq assay has been validated with the new generation IP-Star® Compact Automated Workstation. Furthermore Diagenode's high quality ChIP-seq grade antibodies have been used.
Features:
- Revolutionary: Only 10.000 cells needed for complete ChIP-seq procedure
- Reliable and accurate sequencing library preparation from just picogram inputs
- Validated on the IP-Star® Compact Automated Platform
Best Workflow Practices for ChIP-seq Analysis with Small Samples
Combined chromatin immunoprecipitation and next-generation sequencing (ChIP-seq) has become the gold standard to investigate genome-wide epigenetic profiles, however this method typically requires a large number of cells. This application note describes a method for carrying out ChIP-seq with a small numbers of cells, using the True MicroChIP Kit and the MicroPlex Library Preparation™ Kit from Diagenode and Advanced Analytical Technologies, Inc. (AATI). This enables reliable analysis of rare or difficult-to-obtain cell types.
From Minuscule Amounts to Magnificent Results: Reliable ChIP-seq Data from 10,000 cells with True MicroChIP™ and MicroPlex Library Preparation™ Kits
In this application note from Diagenode, learn about the unique True MicroChIP Kit and the MicroPlex Library Preparation™ Kit, which enable the reliable detection of protein-DNA interactions by ChIP-PCR or ChIP-seq starting from inputs as low as 10,000 cells.
