TransFectin™ Lipid Reagent 0.5 ml by Bio-Rad

Manufacturer Bio-Rad  |   Model: 1703350  |  Available Worldwide
TransFectin reagent is a cationic lipid reagent that delivers the highest efficiencies and expression levels over a broad range of cell lines, including some difficult-to-transfect and primary cells, while minimizing damaging cytotoxic effects observed with other high-efficiency general transfection reagents.

TransFectin™ Lipid Reagent 0.5 ml by Bio-Rad product image
TransFectin™ Lipid Reagent 0.5 ml
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Choose the general purpose TransFectin Lipid Reagent for high efficiency transfection of your cell lines or primary cells, including difficult-to-transfect cells. The proprietary mixture of cationic compound and the co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) is designed to provide high expression levels with reduced cytotoxicity.

  • 0.5 ml is generally sufficient for 60 to 100 cell transfections in 35 mm plates
  • Simple 3-step protocol gives high-efficiency transfection in a broad range of cells with high levels of cell viability
  • Transfection at culture densities from 40% to 90%
  • Designed to work in media containing serum but can be used in the absence of serum
  • No post transfection media changes required
  • Polystyrene is recommended because cationic lipid-plasmid complexes may bind to polypropylene

The Transfection Protocol Online Library contains protocols from a range of sources for transfection using lipids, electroporation and biolistic particle delivery. The library has a comprehensive list of cell lines and primary cultures. Use the information in the library as a starting point for designing protocols for your cells. The TransFectin Lipid Reagent Instruction Manual provides information about optimization as well as changes in protocols when changing from a different cationic lipid.

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TransFectin™ Lipid Reagent 0.5 ml by Bio-Rad product image

TransFectin™ Lipid Reagent 0.5 ml

Manufacturer Bio-Rad  |  Available Worldwide

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