Thermo Scientific™ HisPur™ Ni-NTA Resin by Thermo Fisher Scientific

Manufacturer Thermo Fisher Scientific  |   Model: 88222
4.7
/
5.0
  |  1 reviews
HisPur Ni-NTA Resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.
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Thermo Scientific™  HisPur™ Ni-NTA Resin by Thermo Fisher Scientific product image
Thermo Scientific™ HisPur™ Ni-NTA Resin

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Average Rating: 4.7
1 Scientist has reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money


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    Expert Reviewer
  • Member since: 2020

  • Organization: Montana State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Great product, has made protein cleaning easy
Rating: 4.7

  • Application Area: Protein purification

"Good recovery, made protein purification easy, easy to use and after care."

Review date: 21 Jan 2022 | Thermo Scientific™ HisPur™ Ni-NTA Resin

HisPur Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates, such as those that are attained with Thermo Scientific B-PER Bacterial Protein Extraction Reagents. The resin performs well in batch-binding and spin-column procedures at a variety of scales. Performance equals or exceeds popular Ni-NTA resins from other suppliers. HisPur Ni-NTA Resin is a high-quality, stable and resilient affinity support. Tests confirm that no decrease in performance occurs after at least five repeated uses. These data indicated that the resin is highly resistant to structural degradation or nickel ion leaching during normal use.


Features:

  • High capacity—bind up to 60 mg of 6xHis-tagged protein per milliliter of resin
  • Versatile—purify proteins using native or denaturing conditions
  • Compatible—use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
  • Cost-effective—reuse the same batch of resin at least five times
  • Flexible—available in multiple formats including bulk resin, spin columns, chromatography cartridges and 96-well filter plates

Product Overview

Thermo Scientific™  HisPur™ Ni-NTA Resin by Thermo Fisher Scientific product image

Thermo Scientific™ HisPur™ Ni-NTA Resin

Manufacturer Thermo Fisher Scientific

4.7 / 5.0 | 1 reviews