SsoFast™ EvaGreen® Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)
2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions
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Diagnostic
The SsoFast Evagreen is an evolution of standard sybr-green. Evagreen allows us to achieve a very high fluorescence signal, higher than sybr-green. The master mix is complete with all components; you only have to add primers and water. The thermal profile is fast, we have improve the time of our analysis. We can get results in one hour. We have observed an increase of processivity and sensibility of our assays.
Review Date: 8 Jul 2014 | Bio-Rad
Use SsoFast EvaGreen Supermix for effective performance in a wide range of qPCR applications. Our patented Sso7d fusion polymerase* uses the Sso7d DNA-binding protein to stabilize the polymerase-template complex, increasing processivity and providing greater speed and reduced reaction times compared to conventional DNA polymerases, without affecting PCR sensitivity, efficiency, or reproducibility.
Key Features and Benefits
- Fast qPCR results (in less than 30 min) and high performance
- Minimal inhibition of PCR ensures maximum efficiency, sensitivity, and reproducibility
- Single copy detection of target genes
- Robust discrimination and reproducibility
Applications and Uses
- Dye-based qPCR detection
- PCR and real-time PCR analysis
- Nucleic acid amplification and expression profiling
- Gene expression analysis
- Genotyping studies
- Genomics-related applications
See the SsoFast EvaGreen Supermix product page for more information and documentation.
Related Products
- View all Real-Time PCR Supermixes and Kits
- PrimePCR™ PCR Primers & Assays
Related Categories
- PCR and qPCR Reagents
- Real-Time PCR Detection Systems
- PCR Plastic Consumables
- Instrument Validation Tools and Services
Online Resources
- qPCR/Real-Time PCR
- Genomics
*U.S. patents 6,627,424; 7,541,170; and 7,560,260
SsoFast™ EvaGreen® Supermix Stability for Automated qPCR
Quantitative PCR (qPCR) is a powerful technique for quantitation, mutation analysis, copy number variation, and genotyping studies. Many of these applications increasingly require high-throughput experiments utilizing automated real-time PCR systems. This study evaluates the stability and compatibility of SsoFast EvaGreen supermix for automation applications, demonstrating that the supermix can be used to achieve highly consistent results over a large dynamic range of input templates.
Bio-Rad qPCR Supermixes: Innovative Reagents for Superior Performance
Watch this video to discover Bio-Rad's innovative real-time PCR supermixes. Utilizing advanced formulations and enzyme engineering, they provide optimal real-time PCR data on any instrument, under any conditions, at any time. Bio-Rad PCR supermixes are designed with advanced enzyme and buffer formulations for robust amplification with even the most challenging templates and target sequences.
Improve your qPCR Results with High Processivity Sso7d Fusion Polymerase from Bio-Rad
Watch this video to discover how Bio-Rad’s fusion polymerase has an increased affinity for binding and for the priming site on DNA templates. The double stranded DNA binding domain Sso7d increases processivity through greater binding to the template leading to better handling of challenging samples.
PCR Supermixes: Advanced Enzyme and Buffer Components for Optimal Performance
Watch this video to find out what goes into Bio-Rad’s supermixes. With a diversity of advanced components, Bio-Rad real-time PCR supermixes are ideal for providing optimal data across a broad range of sample types and target regions, including complex sample types. Bio-Rad PCR supermixes are designed with advanced enzyme and buffer formulations for robust amplification with even the most challenging templates and target sequences.
