During the course of menstrual cycle, the sex hormones cause characteristic changes in the vaginal epithelium. The hormone situation can be evaluated with the aid of stained vaginal smears. The Shorr staining is used for hormonal cytodiagnosis only, however the differences between eosinophilia and cyanophilia are more distinct. The ratio of eosinophilic to cyanophilic cells casts light on the effects of the follicular and the corpus luteum hormones. The number of eosinophilic cells increases under the influence of the follicular hormone, whilst that of the cyanophilic cells increases under the influence of corpus luteum hormone. The Shorr staining solution is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes.