Member since: 2017
Organization: Comenius University in Bratislava
Great and unique column.
Application Area: Analysis of drug metabolites in RBC lysates
"Great column, great and unique separation. And if you work with MS it offers a gain in sensitivity in comparison with RP columns. A bit of a downside is short column life. And you have to be careful with purity of things you put on these columns because they are prone to clogging."
Member since: 2016
Organization: Mitchell Cancer institute
Great product, marginal support
"This column is very stable and works great for the HILIC analysis of glycopeptides. Its pressure is very stable. The sequent group however is not very friendly during tech support."
Member since: 2005
Organization: University of Tokyo
Application Area:Plasma analysis
"ZIC-HILIC has good separation property for small polar compounds."
Member since: 2013
Organization: Idexx Labs
Great Column to have and work with...
Application Area:Analyze metabolites from plasma
"SeQuant ZIC-HILIC columns have wide range of pH. I have used it with ease and the results are highly reproducible. I feel that the price can be lowered. "
Member since: 2013
Organization: Department of Analytical Chemistry, University of Valencia
Application Area:Analytical Chemistry
"The column gave good results as expected. The quality of the packed material is really good. The worst thing is the external surface of the container because its made of plastic and it could swell."
ZIC®-HILIC HPLC columns for the separation of all classes of polar and hydrophilic compounds.
What is HILIC?
HILIC or Hydrophilic Interaction Liquid Chromatography is a straightforward chromatographic technique for separation of many types of polar and hydrophilic compounds. To put it simple one can say that HILIC is a normal-phase (NPLC) type of separation but uses reversed-phase (RPLC) type eluents.
In HILIC one has:
Analytes such as amino acids, peptides, carbohydrates, plant extracts and various other polar compounds that usually have little or no retention and may be affected by wettability problems in RPLC, generally have strong retention on the ZIC®-HILIC column.
The zwitterionic ZIC®-HILIC stationary phase is attached to porous silica and is available in columns from capillary to preparative dimensions. The separation is achieved by a hydrophilic partitioning mechanism superimposed on weak electrostatic interactions.