Average Rating: 4.3
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"This is the best DHEA-S kit we have ever used. It is easy to set up and demonstrates excellent reproducibility. The test is relatively quick compared to some other brands we have tested. We are also pleased with the customer service we have received from DRG."Review date:
02 Sep 2014 | Salivary DHEA ELISA
An enzyme immunoassay for measurement of Dehydroepiandrosterone (DHEA) in saliva. PRINCIPLE of the testThe DRG Salivary DHEA ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a polyclonal antibody directed towards an antigenic site of the DHEA molecule. Endogenous DHEA of a sample competes with a DHEA-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of DHEA in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of DHEA in the sample.