RNAscope™ Multiplex Fluorescent Assays by Advanced Cell Diagnostics

The RNAscope® Multiplex Fluorescent assays are ideal for simultaneous detection studies of any genes in nearly any tissue-type using fluorescent labels.


RNAscope™ Multiplex Fluorescent Assays by Advanced Cell Diagnostics product image
RNAscope™ Multiplex Fluorescent Assays
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The RNAscope® Multiplex Fluorescent assays are ideal for simultaneous detection studies of any genes in nearly any tissue-type using fluorescent labels. We offer with various levels of plexing to:

  • FIND cells of interest by using cell specific markers
  • CONFIRM cells by simultaneous detection of additional targets
  • CHARACTERIZE cellular heterogeneity of complex tissues by mapping and identifying multiple cell subtypes simultaneously

Spatial analysis of gene expression is an essential tool for comprehensive studies of complex, highly heterogeneous tissues, such as the brain, tumors, and developing organs. Traditional RNA-ISH can be challenging due to low sensitivity, inconsistent detection accompanied with limited plexing. RNAscope® Fluorescent Multiplex assays provide exceptional sensitivity, allowing single-molecule detection of RNA targets at the single cell level. 

The multiplex assays offer exceptional sensitivity and allow single-molecule detection of up to 12 RNA targets simultaneously. These assays offer a novel and proprietary method of in situ hybridization (ISH) to achieve single cell resolution for RNA detection in a multitude of slide mounted samples. The assays are compatible with various sample types such as fresh frozen, fixed frozen and FFPE. 

  • Single molecule RNA detection.
  • Single cell resolution
  • Sensitive 
  • Specific
  • Spatial Context