Member since: 2020
Organization: Washington State University
One of the best techniques to visualize and quantify RNA molecules in a tissue section.
Application Area: RNA in situ hybridization
"I have used RNAscope V2 (fluorescent) and chromogenic in-situ hybridization assays for spatial detection of target RNA species in plants and animal tissue sections. This technique is amazingly WONDERFUL. Once optimized, this method is straightforward and very easy to use. After sales customer care is one of the best in industry. I had a tough time to optimize the assay due to the nature and type my target tissues, but I got outstanding technical support from ACD team during the standardization process. Results are reproducible if quality of tissues are maintained. Initial investment on RNAscope tools, kit and probe designing is a bit expensive but full value for money. Now RNscope is fully optimized in our lab and we are using it routinely.We published a modified protocol on it (doi: 10.1186/s13007-020-00614-4) and employ this technology for a few other projects too. We got beautiful pictures of multiple target RNAs as per our research objective and now many of our collaborators are interested in RNAscope technology."
The RNAscope® Multiplex Fluorescent assays are ideal for simultaneous detection studies of any genes in nearly any tissue-type using fluorescent labels. We offer with various levels of plexing to:
Spatial analysis of gene expression is an essential tool for comprehensive studies of complex, highly heterogeneous tissues, such as the brain, tumors, and developing organs. Traditional RNA-ISH can be challenging due to low sensitivity, inconsistent detection accompanied with limited plexing. RNAscope® Fluorescent Multiplex assays provide exceptional sensitivity, allowing single-molecule detection of RNA targets at the single cell level.
The multiplex assays offer exceptional sensitivity and allow single-molecule detection of up to 12 RNA targets simultaneously. These assays offer a novel and proprietary method of in situ hybridization (ISH) to achieve single cell resolution for RNA detection in a multitude of slide mounted samples. The assays are compatible with various sample types such as fresh frozen, fixed frozen and FFPE.