RapidFire High Throughput MS
High-throughput LC/MS
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Easy to use and efficient!
Analysis of tissue samples
This instrument is extremely easy to use, it’s accurate and reliable. The customer service was outstanding and when we encountered issues with it, they were very resourceful!
Review Date: 15 Nov 2021 | Agilent Technologies
Easy to use & analyze.
Metabolite analysis in natural products
It is easy to use, but maintenance is needed.
Review Date: 21 Jun 2021 | Agilent Technologies
Great results.
Identify the elements and isotopes of a sample
The instrument is very effective for my application since it produces very high quality and also reproducible results. In my time using it in our laboratory, I have found it to be easy to use since the method of sample preparation is completely adhered to which is not complicated. The rest is just data read out. It is good value for money, the quality and reproducibility of results are worth the money. I would encourage other labs to get this instrument.
Review Date: 25 Jun 2020 | Agilent Technologies
This is most important invention in the field of separations.
Solid-phase extraction of complex sample matrix
This is a high-throughput separation and detection instrument. Using the BenchBot Robot for automated plate handling and solvent switching has given great advantages over automated development and multiple analysis. Critical separation during the use of LC/MS is now replaceable using this ultra-fast direct detection method for a wide range of applications, even in biological matrices.
Review Date: 29 Apr 2020 | Agilent Technologies
Good instrument.
MS analysis
Agilent mass spectrometers gives accurate mass with ease of use and efficiency.
Review Date: 12 Mar 2020 | Agilent Technologies
One high value instrument.
Biomolecule separation from a crude sample preparation
It is so user friendly, easy to learn and adapt. Hasle free knobs and software connects all movable parts with mass spec. Data is so reliable, no one can modify. This product doesn't require any service or maintenance. One can easily reach a service person at any time over the phone. It is indeed a great instrument.
Review Date: 9 Mar 2020 | Agilent Technologies
Most reliable and reproducible instrument for HTS screening.
Large molecule analysis
Reliable instrument for HTS, ceramide analysis. It can also be utilized for quantitative analysis of small molecules.
Review Date: 10 Feb 2020 | Agilent Technologies
The results are great and relaible
Pharmaceuticals
The instrument is very user-friendly. Also easy to maintain.
Review Date: 24 Apr 2018 | Agilent Technologies
Great!!
Microfluiics
This product is the best for the microfluidic experiments.
Review Date: 8 Mar 2017 | Agilent Technologies
Pharmacology
This is an excellent product and superb service. It is the best in the world.
Review Date: 13 Oct 2015 | Agilent Technologies
The Agilent RapidFire 400 is an integrated autosampler for ultrafast sample cleanup based on automated solid phase extraction (SPE) and introduction to mass spectrometry. Designed for highest-throughput laboratories, the large capacity, temperature-controlled, 1536 well-plate handling robot allows unattended weekend operation with sample injection times as short as 2 seconds. RapidFire 400 builds on successful previous models, combining more than 14 years of high-throughput mass spectrometry innovation.
This system provides reliable high throughput and accurate measurements for the qualification of drug targets like automated ADME in discovery, compound binding assessment, elucidation of metabolic processes, the discovery of novel metabolomics targets, and much more. Fully controlled by a single PC, the RapidFire 400 can be combined with Agilent triple quadrupole, time-of-flight, and quadrupole time-of-flight mass spectrometers, while integrating the Ultivo LC/TQ into the system dramatically reduces the footprint of the autosampler and mass spec combination.
Features:
- Fully automated online sample introduction to Agilent triple quadrupole, time-of-flight, and quadrupole time-of-flight mass spectrometers, using a single control software
- Integrating the Ultivo LC/TQ into the RapidFire 400 footprint saves valuable lab space
- Enables different assays in a single batch based on innovative fluidics using proven Agilent 1260 Infinity II quaternary pumps
- Eliminating chromatography reduces sample analysis time from minutes to seconds.
- Large sample capacity with storage for over 130,000 samples in 1536-well plates allows longer unattended runs
- Temperature-controlled sample storage unit stores samples longer without compromising integrity
- Integrated plate handler robotics and barcode reading simplify batch configuration and ensure sample integrity, from loading to analysis
- With analysis times of seconds per sample and SPE cartridges reusable for thousands of samples, solvent use and waste generation are substantially reduced
High-throughput, ion-pairing-free, HILIC analysis of oligonucleotides using Agilent RapidFire coupled to quadrupole time-of-flight mass spectrometry
In this application note, Agilent Technologies describes a high-throughput, ion-pairing-free method for oligonucleotide characterization using the Agilent RapidFire high-throughput MS system.
High-throughput mass spectrometry analysis of synthetic oligonucleotides
In this application note, Agilent Technologies describes and compares two methods, Fast LC and RapidFire, for the high-throughput sampling and desalting of oligos. Each method was optimized for speed on 18mers, then characterized for performance on a range of synthetic DNA and RNA, 18 to 100mer in length.
Automated method development using the Agilent RapidFire High‑Throughput Mass Spectrometry System
Development of new analytical methods to monitor compounds of interest using SPE/MS/MS involves optimizing several parameters. The Agilent RapidFire high throughput MS system’s expanded capabilities allow the user to automate much of this optimization.
In this application note, Agilent Technologies demonstrates a procedure for optimizing a method for a small example molecule, cyclic AMP.
Ultrafast analysis of metabolic stability assays using Agilent RapidFire High-Resolution MS
In this application note, Agilent Technologies evaluates the ability of a RapidFire High-throughput System integrated with an Agilent Q-TOF MS to provide equivalent assay results to LC/MS/MS, but with a much more efficient workflow and higher throughput.
Ultrafast analysis of metabolic stability assays using an Agilent Ultivo Triple Quadrupole LC/MS
In this application note, Agilent Technologies demonstrates the results that can be achieved using the RapidFire integrated with an Agilent Ultivo triple quadrupole LC/MS, while significantly reducing the instrument’s footprint.
High-throughput (sub-2.5 second) direct injection analysis by mass spectrometry
Due to large numbers of samples and the associated analysis time for each, using mass spectrometry (MS) as a primary screening technique can be a long, tedious process.
In this application note, Agilent Technologies presents a modified Agilent RapidFire high throughput MS system (RapidFire) that bypasses SPE desalting and enables a sub-2.5 second sample throughput rate, making the analysis of 35,000 samples a day possible.
High-throughput analysis of nitrosamines using RapidFire coupled to Ultivo Triple Quadrupole
Several highly sensitive quantitative methods have been developed for the analysis of nitrosamines using mass spectrometry. However, these methods rely on chromatographic separations that take several minutes per sample. This application note by Agilent Technologies explores the simultaneous quantitation of a panel of nitrosamines in less than 15 seconds per injection.
A Proven, rapid and complete approach to high-throughput mass spectrometry
A path that offers the chance to increase productivity as much as ten-fold – particularly while reducing the cost per sample – seems like an unmissable opportunity. These are exactly the benefits that can be achieved with the Agilent RapidFire 400: a large-capacity, temperature-controlled, integrated solution for ultrafast data acquisition. Designed for highthroughput laboratories, RapidFire 400 features a plate handling robot that allows for unattended operation, with cycle times as low as 2 seconds per sample. Support for 96-, 384-, and 1536-well plates means the system can store over 130,000 samples for analysis and run all weekend long.
Enhance lab productivity with Agilent automation solutions
Lab productivity is facing significant challenges, including rising demand, staffing shortages, and the need to maintain data quality while achieving high throughput. Discover how Agilent Technologies is transforming lab productivity with advanced automation solutions. In this video, Agilent Technologies’ Senior Product Managers Thomas Wu and Kevin McCann introduce the Bravo liquid handling platform and high-throughput mass spectrometry solutions such as the StreamSelect LC/MS System and RapidFire High Throughput MS. Learn how Agilent’s automation technologies help scientists enhance efficiency, reliability, and scalability in life sciences and genomic applications.
This video was filmed at SLAS2025.
