Q5® Site-Directed Mutagenesis Kit by New England Biolabs Inc.

5.0
/
5.0
  |  2 reviews
Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, room temperature circularization and template removal. Transformation int... Read more


Q5® Site-Directed Mutagenesis Kit by New England Biolabs Inc. product image
Q5® Site-Directed Mutagenesis Kit

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Average Rating: 5.0
2 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Advanced Reviewer
  • Member since: 2013

  • Organization: MSKCC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Best mutagenesis kit for all!
Rating: 5.0

  • Application Area: molecular biology

"Great thing with this kit is: one kit for all. Insertion, point mutation, deletion can all be finished in here. The simplicity, efficiency, short work time are all good."

Review date: 14 Mar 2019 | Q5® Site-Directed Mutagenesis Kit
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: Umass Medical School



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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great kit for site directed mutagenesis
Rating: 5.0

  • Application Area:molecular cloning

"Very easy to use with high-quality results. No limits on plasmid size and very versatile."

Review date: 17 Apr 2018 | Q5® Site-Directed Mutagenesis Kit
Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.

The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, room temperature circularization and template removal. Transformation into high-efficiency NEB 5-alpha Competent E. coli, provided with the kit, ensures robust results with plasmids up to at least 14 kb in length.

Q5® Site-Directed Mutagenesis Kit Features:

  • Non-overlapping primer design ensures robust, exponential amplification, generating a high percentage of desired mutations from a wide range of templates
  • Intramolecular ligation and transformation into NEB high-efficiency competent cells results in high colony yield
  • Extremely low error rate of Q5 Hot Start High-Fidelity DNA Polymerase reduces screening time
  • Hot start polymerase enables room temperature reaction set-up
  • DpnI background reduction permits a wide range of starting template concentrations
  • Use of standard primers eliminates additional expenses from phosphorylated or purified oligos
  • Easy-to-use PCR master mix and unique multi-enzyme KLD mix offer convenience and quality
  • Rapid and direct treatment step proceeds at room temperature in 5 minutes

Q5® Site-Directed Mutagenesis Kit Applications:

  • Difficult PCR
  • Fast PCR
  • High-Throughput PCR
  • High-Yield PCR
  • Long Range PCR
  • Multiplex PCR
  • Routine PCR
  • Site Directed Mutagenesis