Features:
Parameters that Prometheus Panta measures:
Dynamic Light Scattering (DLS)
SIZE ANALYSIS
Tsize (from growth of rH in cumulants fit): Temperature at which average particle size begins to increase due to unfolding
Average scattering intensity: Identifies if sample concentration is too high or low for proper size distribution analysis
CUMULANT OR SIZE DISTRIBUTION/REGULARIZATION ANALYSIS
rH: Hydrodynamic radius shows size of particle in its solvated state
PDI: Polydispersity index represents distribution of size populations within a sample, and is used to find impurities or poorly folded protein preparations
SELF-INTERACTION ANALYSES
kD: The self-association parameter is used as an indication of a protein’s propensity to aggregate at higher concentrations in a given buffer environment
D0 (*derived from kD analysis): Theoretical diffusion constant at concentration = 0
nanoDSF
THERMAL UNFOLDING
Tm (for 330 nm, 350 nm, and ratio): Melting temperature, the point at which 50% of a protein is unfolded; higher melting temperatures indicate greater thermal stability
Tonset/Ton (for ratio): Temperature at which unfolding begins; higher Ton values indicate increased thermal stability, and it is considered favorable to have a sharper slope at the unfolding inflection point, which occurs when the Ton is close in value to the Tm
Ea (*derived from thermal unfolding data): Activation energy of unfolding, a parameter that informs on the stability of a protein in a given buffer environment
Reversibility of unfolding: Point at which aggregated protein cannot be recovered and refolded without other chemical means
Backreflection
AGGREGATION
Tturbidity: Temperature at which large amorphous aggregates (>12.5 nm radius) begin to form in solution
SLS
SIZING INFORMATION
Molecular weight: Average molecular weight of all particles in solution
SELF-ASSOCIATION AND AGGREGATION PROPENSITY
Tscattering: Temperature at which scattering intensity begins to change, indicating increased size and formation of smaller aggregates
B22: Second virial coefficient ,used to extrapolate self-association behavior at higher concentrations
Manufacturer NanoTemper Technologies | Available Worldwide
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