PhenoImager™ HT by Akoya Biosciences
Status:
Reviewer
Member since: 2020
Organization: University of Surrey
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- Ease of use
- After sales service
- Value for money
A game-changer in immunohistochemistry-based research of tumor microenvironments.
Application Area: Analysis of immune markers in tissue - multiplex IHC
"Having the Vectra Polaris in our lab has transformed our ability to analyze and understand the tumor immune microenvironment. Within a year of having this technology we have developed novel 9-plex immune marker panels and applied them to endometrial, bladder and esophageal cancer tissues. PhD students with little to no experience of IHC have quickly mastered the ability to perform multiplex IHC and are also now fully competent at using the software needed to analyze their samples. Before obtaining this technology, we were unable to carry out equivalent immunohistochemistry analyses, both in terms of multiplex staining capability and also quantitative regional analysis of multiplex-stained tissue sections. The increased information and thus understanding of the tumor microenvironments this technology is revealing to us will greatly help inform us in our development of more effective combination immunotherapies. "
Review date: 03 Dec 2020 | PhenoImager™ HT
As part of our PhenoImager™ translational solution, multispectral imaging on the PhenoImager HT can be applied across a whole slide using the 6-plex, 7 Color Opal Polaris reagent kit. This enables the biology to be explored at multiple scales, from cell-to-cell interactions to the macroscopic tissue architecture.
The whole slide multispectral imaging capability creates a simpler and more robust workflow as fields of view do not need to be selected eliminating selection bias. You also retain a whole slide record, no re-scans required, so that you can easily re-analyze imagery as new understanding emerges.
Tissue sections or TMAs can be labeled with immunofluorescent (IF) or immunohistochemical (IHC) stains such as Opal™, or with conventional stains such as H&E and trichrome.
When using IF or IHC stains, multiple proteins can be measured on a per tissue, per cell, or per cell compartment (e.g. nuclear, cytoplasmic) basis – even when signals are spectrally similar, are located in the same cellular compartment or are obscured by autofluorescence.
Product Overview
