Member since: 2019
Organization: Centre for Forensic Programmes and DNA studies, University of Benin
Application Area: PCR for DNA extraction to elongate the fragments of the extracted DNA
"Excellent for performing polymerase chain reaction to elongate the extracted DNA."
PCR Polishing Kit enables an increase in blunt-ended cloning efficiencies associated with polymerase chain reaction (PCR)-generated fragments. It polishes the ends of the 3’ overhang extensions of polymerase-generated DNA fragments directly from PCR amplification and can completely fill in 5’ overhangs to generate blunt ends.
Dramatic increases in the population of blunt-end DNA fragments following polishing with Agilent’s PCR Polishing Kit enables significant increase in overall experimental efficiency associated with procedures utilizing blunt-ended ligation.
For Research Use Only. Not for use in diagnostic procedures.