Member since: 2020
Organization: The Florey Institute of Neuroscience and Mental Health
An amazingly versatile platform for all your electrophysiological needs.
Application Area: Ion channels and human diseases
"Our research focuses on understanding the pathology of ion channel disorders, using in vitro and in vivo electrophysiology to reveal opportunities for developing novel therapies. In particular, our research focuses on ion channel gene mutations that cause epilepsy. Patchliner has been an invaluable tool enabling us to examine ion channels both endogenously and exogenously expressed by a variety of different cell types, including mammalian cell lines as well as primary cells. The system allows us to examine the effects of different mutations and different compounds on the biophysical properties of a variety of ion channels, including sodium, potassium and calcium, to mention a few. Patchliner produces high fidelity, reproducible data which is very comparable to that produced using conventional techniques. It is easy to use and throughput is an attractive bonus. The systems temperature control is a really great feature for temperature sensitive channels. The after sales service from this company is second to none."
Member since: 2020
Organization: Bionomics Ltd, Australia
Flexible research tool and screening workhorse for ion channels.
Application Area:Drug discovery
"Patchliner is an automated, single pipette, 8 parallel-channel system. It comes with many, easily modifiable, protocols for all ion channels groups. I have used Patchliners for primary, secondary and selectivity screens in voltage and ligand gated ion channel programs. The quality of the recordings is comparable to the manual patching, even for fast ligand gated channels such as alpha 7 nicotinic acetylcholine receptor. The technical support from Nanion is excellent. Love it."
Member since: 2020
Organization: Philip Morris Products SA
Overall the Patchliner is a high quality machine supported by high quality people!
Application Area:Ion channel pharmacology
"The Patchliner comes with robust and high quality Heka amplifiers. Therefore a previous knowledge of the Heka software is clearly helpful. Nevertheless, for the people that are not familiar with the Heka software, Nanion Patchliner created their own software, with a more friendly user interface, to control the Heka commands. Overall it is easy to use. The Patchliner offers the possibility to measure up to 8 cells simultaneously (some cells are "easier" than other though and the success rate can vary accordingly). The solutions are directly applied by the Patchliner "Syringe Pipettor". The speed of the solution application can be modified and the protocols are very flexible and easy to set up. Results are very reproducible. It allows the recording of all types of ion channels (including mechanosensitive channels!). In addition, the Patchliner support team is incredible to help you anytime you need in a very short notice!"
Member since: 2019
Fantastic team and equipment.
Application Area:Ion channel electrophysiology
"Exceptional after sale care from the team at Nanion. Always available for an email or call. Always happy to do traning and help new members of the team learn how to use complex software."
The Patchliner is versatile yet robust, ideal for basic research of ion channel biophysics and mechanisms of action, and sophisticated assays including heat activation of TRP channels, activation of Calcium-activated channels by internal exchange and fast external solution exchange with minimal exposure for ligand-gated ion channels such as nAChα7. The Patchliner is also an excellent tool for routine assays such as safety screening of hERG or other cardiac ion channels in line with the CiPA initiative.