OneTaq® DNA Polymerase
OneTaq DNA Polymerase is an optimized blend of Taq and Deep VentR™ DNA polymerases for use with routine and difficult PCR experiments. The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.OneTaq DNA…
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Great results after using this product
Molecular Biology
The product is a high-fidelity product, and easy to handle. The product is also reliable in all my experiments.
Review Date: 10 Feb 2023 | New England Biolabs Inc.
Robust and cost-effective Taq Polymerase.
Routine PCR
This is a highly useful cost-effective enzyme. Results are reproducible and meet our needs for the robustness of the application. Note that OneTaq has an extension Tm of 68 degrees C and not 72 degrees C. NEB provides fantastic technical assistance.
Review Date: 30 May 2019 | New England Biolabs Inc.
OneTaq DNA Polymerase is an optimized blend of Taq and Deep VentR™ DNA polymerases for use with routine and difficult PCR experiments.
The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.
OneTaq DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions containing OneTaq GC Reaction Buffer.
OneTaq® DNA Polymerase Features:
- Ideal for routine, AT- and GC-rich PCR
Applications:
- High Sensitivity PCR
- High Throughput PCR
- Routine PCR
- GC-rich PCR
- AT-rich PCR
- Colony PCR
- Long PCR (up to ~6 kb genomic)
NEB Product Portfolio for Genomics, Proteomics and Cell Biology
This video presents New England Biolab’s product portfolio of restriction enzymes, recombinant polymerases, and modifying enzymes for a wide range of applications, from next generation sequencing to protein purification.
New England Biolabs: How to Amplify GC Rich DNA?
Watch this video from New England Biolabs to learn how OneTaq and Q5 DNA polymerases optimize the amplification of AT rich and GC rich DNA targets respectively.
