Odyssey® Loading Indicators

Odyssey Loading Indicators (OLI) provide a simple, convenient method to evaluate the consistency of sample loading volume across gel lanes, as well as the uniformity of Western blot transfer. Each Odyssey Loading Indicator is a single 28 kDa recombinant protein directly labeled with a near-infrared fluorescent dye. You can detect the 700 nm version of OLI in the 700 nm channel of your imaging system and the 800 nm version of OLI in the 800 nm channel.

• Evaluate Consistency. Ensure samples were equally loaded in each lane
• Check Uniform Transfer. Confirm that transfer from gel to membrane was uniform
• Assess Stable Expression. Use OLI as part of the process to determine if a housekeeping protein is stably expressed and can be used for Western blot normalization