Normal Serums & Gamma Globulins by Jackson ImmunoResearch Laboratories Inc.

Manufacturer Jackson ImmunoResearch Laboratories Inc.  |  Available Worldwide
4.5
/
5.0
  |  2 reviews
Normal serum can be used as a blocking agent to reduce background. Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze-dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking agent to reduce background from non-specific, conserved-sequence, and/or Fc-receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate in... Read more


Normal Serums & Gamma Globulins by Jackson ImmunoResearch Laboratories Inc. product image
Normal Serums & Gamma Globulins
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Average Rating: 4.5
2 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2018

  • Organization: Yale University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great blocking
Rating: 4.7

  • Application Area: Immunohistochemistry

"Effective blocking for immunochemical assays, especially for IHC and ICC, to reduce background."

Review date: 22 Jan 2018 | Normal Serums & Gamma Globulins
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UMF Carol Davila



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
Very good results, I recommend it
Rating: 4.3

  • Application Area:Analyse metabolites in tissue samples

"Reproducible results very good. Quality of the product very good. Easy to use. Money: it is a bit pricey but I can t complain. I also recommended it to other labs in our department."

Review date: 08 Aug 2016 | Normal Serums & Gamma Globulins

Normal serum can be used as a blocking agent to reduce background.

Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze-dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking agent to reduce background from non-specific, conserved-sequence, and/or Fc-receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.

Gamma globulins are further purified from non-immunized animal serums by salt fractionation, ion-exchange chromatography, and gel filtration. Gamma globulins are an inexpensive source of IgG with only trace amounts of other immunoglobulins and/or non-immunoglobulin serum proteins. Gamma globulins are supplied as sterile liquids in phosphate buffer without stabilizers or preservatives.