Member since: 2018
Organization: Yale University
Application Area: Immunohistochemistry
"Effective blocking for immunochemical assays, especially for IHC and ICC, to reduce background."
Member since: 2016
Organization: UMF Carol Davila
Very good results, I recommend it
Application Area:Analyse metabolites in tissue samples
"Reproducible results very good. Quality of the product very good. Easy to use. Money: it is a bit pricey but I can t complain. I also recommended it to other labs in our department."
Normal serum can be used as a blocking agent to reduce background.
Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze-dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking agent to reduce background from non-specific, conserved-sequence, and/or Fc-receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.
Gamma globulins are further purified from non-immunized animal serums by salt fractionation, ion-exchange chromatography, and gel filtration. Gamma globulins are an inexpensive source of IgG with only trace amounts of other immunoglobulins and/or non-immunoglobulin serum proteins. Gamma globulins are supplied as sterile liquids in phosphate buffer without stabilizers or preservatives.