Solid-Phase Immunoadsorbent Gels
Solid-Phase Immunoadsorbent Gels. Highly purified IgG from the serum of non-immunized animals is coupled to cyanogen bromide-activated 4% agarose gels for use in preparing affinity-purified antibodies or removing cross-reactive antibodies. Proteins are coupled at a concentration of 1 mg protein per ml of settled gel, and are packaged in phosphate buffered saline with sodium azide.
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Solid-Phase Immunoadsorbent Gels.
Highly purified IgG from the serum of non-immunized animals is coupled to cyanogen bromide-activated 4% agarose gels for use in preparing affinity-purified antibodies or removing cross-reactive antibodies. Proteins are coupled at a concentration of 1 mg protein per ml of settled gel, and are packaged in phosphate buffered saline with sodium azide.
Western Blot Troubleshooting Guide
Western blotting is a staple technique of the molecular biology lab. The robust nature of the antigen-antibody interaction allows the presence of specific proteins and peptides to be detected from complex mixtures. If the Western blot is not behaving as expected, the following troubleshooting guide may help isolate the problem.
