Whole IgG Affinity-Purified Secondary Antibodies
Whole IgG antibodies are isolated as intact molecules from antisera by affinity chromatography.
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Using from 10 year's and yes it's great
Quality product..
Provides quality secondary antibodies that are highly affordable with more conjugation options than any other vendor I'm familiar with. It has great quality and it never disappoint. Great tech support as well
Review Date: 31 Jul 2022 | Jackson ImmunoResearch Laboratories Inc.
Great product with good immunological affinity.
Immunology
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins.
Review Date: 31 Mar 2021 | Jackson ImmunoResearch Laboratories Inc.
Excellent results every time.
Immunofluorescence
Jackson's secondary antibodies and antibody fragments are always of the highest quality and work perfectly the first time, every time. I've used them for over 30 years now and have never been disappointed.
Review Date: 1 Jul 2020 | Jackson ImmunoResearch Laboratories Inc.
Quality products with excellent tech support
Immunohistochemistry/IF
Jackson provides quality secondary antibodies that are highly affordable with more conjugation options than any other vendor I'm familiar with. We use both their biotin- and fluor-conjugated antibodies routinely for IHC & IF and have never experienced any issues. Not only do the antibodies perform well, but the technical staff is exceptional; I have learned much from them over the years. They're definitely my go-to for secondary antibodies.
Review Date: 25 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
The quality of antibodies is good.
Cancer research
I have a limited number of antibodies from Jackson, but the quality of these antibodies is good.
Review Date: 24 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
GREAT PRODUCT!
FLOW CYTOMETRY
GREAT PRODUCT! It has shown reproducible results for application in flow cytometry staining.
Review Date: 23 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Perfect results, reliable functionality, rich offer
Immunohistochemistry
I achieve perfect results. Antibodies are easy to use. Dilution for majority of the antibodies is the same that I appreciate. Rich offer of different antibodies, inc. various subclasses. Unfortunately, I miss e.g. secondary anti-rat IgG2a antibody.
Review Date: 23 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Excellent quality and good value for money.
Testing of antibodies on sections of animal tissues
We use already for at least twenty five years the secondary antibodies of Jackson ImmunoResearch Laboratories. We use the IgG purified antibodies but also the other types of antibodies supplied by them. The quality of the products is excellent and they are easy to keep in the -20°C freezer. Because they can be kept liquid at -20°C by adding glycerol there is no danger of degrading. We are very satisfied and they are good value for money.
Review Date: 23 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Great products
Immunostaining of brain sections
We have been using Jackson secondary antibodies for years. They are also good.
Review Date: 22 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Great staining results with minimum nonspecific background
Immunohistochemistry and immunofluorescence
Great quality product with minimum non-specific background. Worth the money spend
Review Date: 22 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Suitable for the majority of immunodetection procedures.
- Detect mouse, rabbit, rat, human, goat, hamster, bovine, cat, chicken, dog, guinea pig, horse, sheep and swine antibodies.
- Comprehensive specificity options including, heavy and light chain, Fc specific, F(ab')2 specific
- Anti-Mouse IgG subclass specific antibodies
- Light-chain specific antibodies for western blotting after immunoprecipitation
- Anti-Digoxin, anti-biotin, anti-FITC and anti-HRP for signal enhancement
- Fluorescent conjugates covering commonly used excitation sources and filter sets, including Alexa Fluors®, DyLight™, Cy™ Dyes, FITC and more.
- Biotinylated and enzyme conjugates (HRP, Alkaline Phosphatase)
- R-PE, APC and PerCP conjugates for flow cytometry
- Alexa Fluor® 680 and 790 conjugates for high sensitivity western blots
- Colloidal gold complexes for electron and light microscopy
Choosing the right reagents for lateral flow immunoassays
In this application note, Jackson ImmunoResearch presents a suite of tools to facilitate the development and manufacture of accurate and sensitive lateral flow immunoassays for point of care (POC) use. Lateral Flow Immunoassays (LFIA) rapid tests are recognized as a powerful tool for point-of-care (POC) immunodiagnostics. They provide a format for the rapid detection and measurement of a variety of agents such as immunoglobulins (Igs) produced in response to infection or allergy, biomarkers such as human gonadotropin for pregnancy testing, food contaminants, substances generated by genetically modified crops, environmentally hazardous chemicals, and illicit drugs. LFIAs are a vital component in assessing health and safety for modern life.
Optimize your IHC and IF for experimental success
Immunohistochemistry (IHC) is a powerful technique used to detect and visualize proteins of interest within a cell or tissue using the specificity of the antigen-antibody reaction in combination with reporter molecules. Flow cytometry, enzyme-linked immunosorbent assays (ELISA) and western blotting are other widely used assay methods that are based upon the principle of antibody-antigen binding.
This application eBook presents a practical guide on how to increase specificity and enhance your labeling technique to achieve optimum lab success, including expert insight and guidance on:
- Control and blocking reagents
- Effective solutions to tackle species on species labeling
- The importance of understanding cross-reactivity and cross-adsorbed secondary antibodies
Chromogenic detection for western blot, IHC, and ELISA
Chromogenic substrates can be used in a number of immunohistochemical applications from staining tissue with IHC through to Western blotting. The chromogenic substrate is added to a blot or tissue previously incubated with an enzyme-conjugated antibody (typically horseradish peroxidase (HRP) or alkaline phosphatase (AP)), which converts the substrate to a colored precipitate. Colorimetric detection is easy to use, although it may require optimization or additional staining to improve signal over background in samples expressing low antigen.
Secondary Antibodies for VHH Discovery
In this application note, Jackson ImmunoResearch Laboratories presents anti-alpaca IgG, subclass, and VHH domain secondary antibodies to optimize VHH antibody development and application.
Cross-Adsorbed Secondary Antibodies and Cross-Reactivity
This application note provides an overview of the unwanted effects of cross-reactivity and cross-adsorption and explains the importance of secondary antibodies with minimal cross-reactivity.
Guide to Multiple Labeling with Secondary Antibodies
Jackson ImmunoResearch provides secondary antibodies with minimal cross-reactivity for multiple labelling. This poster provides an example of a multiple-labelling protocol, using Jackson ImmunoResearch reagents. If you require a hard copy of the poster, please email marketing@jacksonimmuno.com.
Western Blot Troubleshooting Guide
Western blotting is a staple technique of the molecular biology lab. The robust nature of the antigen-antibody interaction allows the presence of specific proteins and peptides to be detected from complex mixtures. If the Western blot is not behaving as expected, the following troubleshooting guide may help isolate the problem.
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