In all myTXTL® kits, gene transcription (TX) and translation (TL) is executed in a single reaction tube utilizing the endogenous TXTL machinery from E. coli. Gene expression is initialized by simply adding the nucleotide template to the myTXTL® Master Mix and recombinant proteins can be detected after only a few minutes. Due to the lack of cell walls and the independency from cell growth and viability, cell-free systems allow rapid and parallel production of soluble or toxic proteins and other complex biological structures under open-reaction conditions. This enables facile manipulation and straightforward downstream processing of synthesized material.
The myTXTL® technology is well-characterized and has been employed for various applications in protein engineering and synthetic biology applications. myTXTL® Sigma 70 Master Mix is the perfect choice for high-yield production of soluble and membrane proteins, rapid prototyping of gene networks using plasmid DNA and RNA, the construction of synthetic minimal cells and to study cellular biology.
myTXTL® unites an extremely user-friendly and safe, GMO-free, handling with exceptional protein synthesis efficiency and great versatility, which makes it similarly well-suited for cutting-edge research and student education.