Monarch® Total RNA Miniprep Kit by New England Biolabs Inc.

Manufacturer New England Biolabs Inc.  |   Model: T2010S  |  Available Worldwide
5.0
/
5.0
  |  1 reviews
Purifies up to 100 µg of high quality total RNA from multiple sample types - using only one kit


Monarch® Total RNA Miniprep Kit by New England Biolabs Inc. product image
Monarch® Total RNA Miniprep Kit

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Average Rating: 5.0
1 Scientist has reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2018

  • Organization: Deutsches Krebsforschungszentrum



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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The only RNA extraction kit you will ever need!
Rating: 5.0

  • Application Area: Extraction of total RNA from cultured cells

"For our applications (i.e. RNA extraction from cultured cells), this kit works like a charm. Streamlined, easy to use, convenient, because all the components (buffers, columns, etc.) are available separately, and cheaper than the competitor in our lab (Qiagen) - this kit gives us all we need. RNA was successfully used for mRNA RNA Seq, small RNA Seq and RT-qPCR, we could even do some targeted sequencing of the DNA isolated with the gDNA removal columns."

Review date: 14 Jan 2020 | Monarch® Total RNA Miniprep Kit

The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, tough-to-lyse samples, such as bacteria, yeast, and plant, can be processed with additional steps that enhance lysis. Cleanup of enzymatic reactions or purification of RNA from TRIzol® - extracted samples is also possible using this kit. Purified RNA has high quality metrics, including A260/280 and A260/230 ratios >1.8, high RIN scores, and minimal residual gDNA. Captured RNA ranges in size from full-length rRNAs down to intact miRNAs. Additionally, differential binding conditions allow selective capture or exclusion of the sub-200 nucleotide RNA pool that includes miRNA, 5S rRNA, and tRNA. Purified RNA is suitable for downstream applications, such as RT-qPCR, cDNA synthesis, RNA-seq, Northern blot analysis, etc.

 

  • For use with blood, cells and tissues
  • Also works with tough to lyse samples (bacteria, yeast, plant)
  • Effectively purifies total RNA of all sizes, including small RNAs >20 nt
  • Efficient genomic DNA removal (column and DNase I-based)
  • Contains Proteinase K for processing of tissues and blood samples
  • Includes RNA Protection Reagent for sample preservation

Product Overview

Monarch® Total RNA Miniprep Kit by New England Biolabs Inc. product image

Monarch® Total RNA Miniprep Kit

Manufacturer New England Biolabs Inc.  |  Available Worldwide

5.0 / 5.0 | 1 reviews