The vast amount of host DNA compared to microbial DNA in clinical samples often reduces the sensitivity in molecular analyses of microorganisms.
MolYsis™ Basic kits are used prior to DNA isolation to deplete the host DNA and enrich the intact pathogen cells from small and large sample volumes. This leads to increased analytical sensitivity and can be combined with any commercial DNA extraction and isolation kits, manual or automated.
With MolYsis™ Basic human or animal cells are selectively lysed and free DNA is degraded in a DNase treatment step. Afterwards a reagent, BugLysis, is used for the broad-range lyses of Gram-positive and Gram-negative bacteria and fungi. The enriched microbial DNA can be isolated and purified with the method of choice.
Applications comprise next generation sequencing, amplicon cloning, real-time PCR, Sanger sequencing and DNA array analysis of microorganisms.
MolYsis™ Basic, MolYsis™ Basic5 and MolYsis™ Basic10 are designed for small (0.2ml), medium (<1-5ml) and large (5-10ml) sample volumes and are validated for a variety of human and animal body fluids.
The MolYsis™ technology is also available in the following products: