MolYsis™Basic by Molzym GmbH & Co. KG

Host DNA depletion for extraction of enriched bacterial and fungal DNA with your own DNA isolation system


MolYsis™Basic by Molzym GmbH & Co. KG product image
MolYsis™ Basic5 kit
Request Pricing

Receive your quote directly from the manufacturer.



0 Scientists have reviewed this product


Write the First Review

No Reviews

The vast amount of host DNA compared to microbial DNA in clinical samples often reduces the sensitivity in molecular analyses of microorganisms. 

MolYsis™ Basic kits are used prior to DNA isolation to deplete the host DNA and enrich the intact pathogen cells from small and large sample volumes. This leads to increased analytical sensitivity and can be combined with any commercial DNA extraction and isolation kits, manual or automated. 

With MolYsis™ Basic human or animal cells are selectively lysed and free DNA is degraded in a DNase treatment step. Afterwards a reagent, BugLysis, is used for the broad-range lyses of Gram-positive and Gram-negative bacteria and fungi. The enriched microbial DNA can be isolated and purified with the method of choice.

Applications comprise next generation sequencing, amplicon cloning, real-time PCR, Sanger sequencing and DNA array analysis of microorganisms.

Benefits:

  • Removal of non-target, human or animal DNA 
  • Selective enrichment of bacteria and fungi
  • Validated for a broad-range lysis of bacteria and fungi
  • DNA-free reagents and consumables
  • Suitable for fluids of 0.2-10ml sample volumes
  • Can be combined with any DNA isolation system of choice

MolYsis™ Basic, MolYsis™ Basic5 and MolYsis™ Basic10 are designed for small (0.2ml), medium (<1-5ml) and large (5-10ml) sample volumes and are validated for a variety of human and animal body fluids. 

The MolYsis™ technology is also available in the following products:

  • MolYsis™ Complete: host DNA depletion and microbial DNA isolation for liquid samples
  • Ultra-Deep Microbiome Prep: as MolYsis™ Complete with additional protocol for tissues
  • SepsiTest™-UMD CE IVD: all-in-one solution for clinical routine including PCR analysis
  • MolYsis-SelectNA™plus: Automated microbial DNA enrichment and isolation
  • Micro-Dx™ CE IVD: Automated microbial DNA enrichment and isolation including PCR analysis