iQue® 4-Plex Apoptosis Kit
Cell Based Assays
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The iQue® 4-Plex Apoptosis Kit is a multi-parameter assay consisting 4 different apoptosis measurements per well (cell viability, caspase 3/7 activity, annexin V binding, and mitochondrial depolarization) for a robust determination of apoptosis progression and insight into mechanism of action.
The individual reagent components are also available separately for greater flexibility.
Key Benefits:
- No wash protocol – just add and read for fast, easily automated workflow.
- Our 4-plex kit is easier to run than some single endpoint kits!
- Four parameters to deliver rich content, including insight on mechanism of action.
- The 4 components are available separately so you can pick and choose.
- Multiplex with QBeads® PlexScreen kits for richer content.
Development of a No-Wash 4-Plex Apoptosis Screening Kit and Validation for Phenotypic Screening
In this application note, Intellicyt reports on the development of a 4-plex apoptosis screening assay in a no-wash format. Validation of the assay performance using Jurkat as the cell model yielded z’ values of > 0.8 for all endpoints, demonstrating broad applicability of the assay for phenotypic screening campaigns as well as toxicity profiling.
Advancing Therapeutic Antibody Discovery with Multiplexed Screening
Antibody-based therapeutics are the fastest growing and most successful therapeutic modality for treating diseases such as cancer, cardiovascular disease, autoimmune disorders and infectious disease.This whitepaper describes how the iQue Screener is used to perform multiplexed screens for antibody binding to either cell surface or to circulating target antigens. The whitepaper also discusses the use of the same platform to carry out high content assays to evaluate the effects of lead candidates in multiplexed cell based and secreted protein assays.
