iQue® Mouse IgG Type and Titer Assay Kit
A patented high-throughput, multiplexed assay kit, with a wide dynamic range requiring no sample dilution or wash steps.
Mouse IgG Type and Titer Assay Kit
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The iQue® High-Throughput Screening (HTS) Cytometry Platform has a novel solution to disrupt the traditional antibody discovery workflow. Success of antibody-based block-buster therapeutics for autoimmune diseases, inflammatory diseases and immune-oncology have accelerated high stakes monoclonal antibody (mAb) discovery—a time-consuming process in which therapeutic antibody candidates are initially generated using hybridoma technology or primary B cell screening after antigen immunization.
The primary screens identify clones with specific attributes (binding specificity, cross species reactivity, selectivity and affinity). Potential candidates from the screen are then assessed for a variety of critical parameters for lead molecule generation such as IgG isotyping, antibody quantification, and cell number/health.
This process typically involves single-endpoint assays requiring sample dilution and multiple washes. That all changes with the iQue® Mouse IgG Type and Titer Kit, a patented high throughput, multiplexed assay with a wide dynamic range requiring no sample dilution or wash steps. The simple mix-and-read workflow simultaneously measures five endpoints.
- Enables quick differentiation between monoclonal and polyclonal wells
- Determines IgG quantity for qualifying stable clones and for downstream functional assays
- Provides IgG isotype information to facilitate primer design for PCR-mediated gene cloning
- Monitors both cell proliferation and cell health prior to RNA isolation prior to PCR-mediated cloning
The Mouse IgG Type and Titer assay is part of the iQue® HTS’ integrated antibody discovery platform technology. The iQue® HTS Platform can acquire data in as little as 20 minutes per 384-well plate, while iQue Forecyt® software provides powerful data mining tools for large hybridoma/B-cell screening studies.
The iQue Forecyt®’s Panorama feature provides interactive and visual multi-plate data analysis including profile maps with user-specified criteria to quickly identify samples containing the desired IgG isotype, antibody concentration and cell health, critical for analyzing data from large, multi-plate screening campaigns.
The total time from samples to actionable data is less than 100 minutes.
Brochures
How to Save Time with the Mouse IgG Type and Titer Kit
Learn how the Mouse IgG Type and Titer assay, a patented novel assay platform for mouse antibody discovery, can save you precious time by combining three separate assays into a single multiplex, high-throughput assay.
iQue® Mouse IgG Type and Titer Kit: A novel solution to expedite antibody discovery
With breakthroughs in molecular engineering and antibody humanization, monoclonal antibodies (mAbs) are one of the fastest-growing classes of biopharmaceuticals for multiple clinical indications. In this application note, Sartorius describes how the iQue® Mouse IgG Type and Titer Kit can be used as an antibody discovery solution that provides a streamlined workflow to reduced the time from data to actionable decisions.
Mix-and-Measure Assay for Screening Hybridoma Supernatants on IntelliCyt Platforms
Antibody engineering is critical to development of new therapies in many fields such as oncology, where monoclonal antibodies are currently the most widely used immunotherapy for cancer. Screening hybridoma libraries for antibodies that bind to cell surface antigens is a time and resource intensive process. The current screening workflow often involves several individual testing steps, including different biochemical tests that test for binding, specificity, followed by cell based assays. In addition to being costly and laborious, purified protein assays such as ELISA that utilize denatured antigens can generate false negatives - potential missed hits that would have bound to antigens in their native conformation. IntelliCyt has developed a “mix-and-measure” intact cell assay for primary screening that simultaneously assesses antigen binding and cross-reactivity.
Intellicyt Mouse IgG Type and Titer Assay Kit: A Novel Solution to Expedite Antibody Discovery
This application note explains how the Intellicyt Mouse IgG Type and Titer Assay Kit can expedite and simplify your antibody discovery workflow, greatly reducing the time to actionable results.
