Impact™ Protein Precipitation Plates

Sample preparation is crucial in achieving desired LC or GC analytical results. Sample matrix effects can result in an array of interferences which can lead to poor chromatography as well as instrumentation drawbacks, hindering your approach and goal for the analysis.

In this user guide, Phenomenex Inc provides all the information you need to choose and use the best sample preparation technique for your work, whether it's filtration, QuEChERS, SLE, PPT, PLR, or SPE. Download your free copy below to read recommendations for your industry, general methods, protocols for easy method development, and much more.

The world is changing and we need to widen our Environmental Scope. Although analytical techniques and established regulated methods are not easily changed; more complex targets, larger lists, and a variety of sample matrices create a daily challenge for environmental chemists. By using the latest technologies and techniques in HPLC/ UHPLC and Sample Preparation, laboratories and chemists are able to develop better approaches to make their work Faster, Cheaper and Easier. Phenomenex is committed to supporting the industry through a comprehensive product portfolio of Sample Preparation, HPLC/UHPLC, and LC-MS, columns and accessories, along with application and method development services to meet industry needs.

This clinical notebook contains a variety of 36 application notes, covering topics in endocrinology and biomarker research, therapeutic drug monitoring, toxicology and sample preparation.

This application note describes Phenomenex’s protein precipitation plate capabilities to rapidly filter and precipitate a large number of samples simultaneously. This enables fast analysis, ensures that samples are clean and reduces the number of filtration steps.

As the determination of vitamin levels in patients becomes an increasingly important factor in the monitoring and diagnosis of diseases, high-throughput laboratories must adopt a rapid and robust method to accurately analyze and quantitate vitamins and their derivatives. This application note demonstrates a method developed by Phenomenex that can be easily automated, can be used in high-throughput labs, and provides sensitivity, speed, and reproducibility.

Thiamine monophosphate (TMP) and thiamine were extracted from human plasma and human breast milk by performing a rapid protein precipitation using Impact Protein Precipitation Plates followed by HPLC analysis using a Gemini 3 μm NX-C18 100 x 3.0 mm HPLC column with fluorescence detection. Impact technology offers easy, fast protein removal while providing maximized recovery of the target analytes. The Gemini 3 μm NX-C18 HPLC column produced excellent chromatographic resolution, sensitivity, and high peak capacities.

In this application note, nicotinic acid and nicotinamide were extracted from human plasma by performing a rapid protein precipitation using Impact Protein Precipitation Plates followed by HPLC analysis using a Gemini 3 μm C18 100 x 4.6 mm HPLC column and positive polarity ESI LC/MS/MS system. Impact technology offers easy, fast protein removal while providing maximized recovery of the target analytes. The Gemini 3 μm C18 HPLC column produced excellent chromatographic resolution, sensitivity, and high peak capacities.

Beta-glucuronidase is a 332 kDa enzyme that remains solubilized after incubation. Centrifuging samples post-hydrolysis is a common step to help remove the enzyme before analysis. This process works well in tubes, but the current centrifuges available for 96-well plates do not allow for sufficient speed to precipitate the enzyme. The increase in popularity of the 96-well plate format for high-throughput analysis has created a need for a simple technique to remove the enzyme that does not add significant cost to the assay. This application note demonstrates a simple post-hydrolysis step that removes the enzyme and significantly improves column lifetime.

Phenomenex’s sample preparation product manager shares his expert advice on preparing challenging biological sample matrices for LC-MS/MS