Hyperphage M13 K07ΔpIII (1x2mL) by PROGEN – passion for research

Manufacturer PROGEN – passion for research  |   Model: PRHYPE-XS
Provides helper phage function in packaging a common phage display phagemid. Infection of bacteria via pIII.


Hyperphage M13 K07ΔpIII (1x2mL) by PROGEN – passion for research product image
Hyperphage M13 K07ΔpIII (1x2mL)
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An Effective Tool for the Isolation of Recombinant Antibodies, Proteins or Peptides from Hyperphage-Packed Libraries.

Introduction

A helper phage technology (""Hyperphage System"") was developed by Rondot et al. (Nature Biotechnology 19:75-81, 2001). The Hyperphage System allows to improve antibody presentation in phage display by increasing the number of antibodies displayed per phage particle (up to 5 vs. 0.01) and thereby the system offers great advantage in the fields of functional gene analysis and proteomics. Panning of phages can be performed with small amounts of antigen and higher efficiency. For example, the application of universal libraries for antibody isolation can be improved by employing panning of hyperphage-packed libraries on blots of protein spots after 2-dimensional gel electrophoresis.

The Hyperphage System

Hyperphages carry a deletion in the pIII gene. They are generated by an E. coli packaging cell line producing functional pIII which is used to package a phage genome with a pIII deletion. The resulting hyperphages carry functional pIII on their surface but lack the pIII gene in their genome. These hyperphages can then be used to infect bacteria with a phagemid library. Each of the resulting display phages carries several copies of the antibody or peptide on its surface, thus dramatically increasing panning efficiency.
  
Protocols
Hust M., Frenzel, A., Tomszak, F, Kugler, J  Dubel, S. (2014) Antibody Phage Display. In: Dubel, S. Reichert, J.M. (eds.) Handbook of Therapeutic Antibodies, 2nd ed. Wiley-VCH, Weinheim, ISBN 978-3-527-32937-3 p. 43-76
Hust, M., Frenzel, A., Schirmann, T. and Dubel, S. (2014) Selection of recombinant antibodies from antibody gene libraries. Methods Mol Biol. 1101, 305-320.2
Hust, M., Frenzel, A., Meyer, T., Schirmann, T. and Dubel, S. (2012). Construction of human naive antibody gene libraries. In: Antibody Engineering: Methods and Protocols, Ed: Chames, P., Meth Mol Biol 907, 85-107
Breitling, F., Broders, O., Helmsing, S., Hust, M Dubel, S (2010) Improving phage display throughput by using Hyperphage, miniaturised titration and pVIII (g8p) ELISA. In: Antibody Engineering (2nd ed) Springer Protocols. Vol. 1, ISBN 978-3-642-01143-6, p. 197-206
Broders, O., Breitling, F., and Dubel, S. (2003). Hyperphage - Improving Antibody Presentation in Phage Display. Methods Mol Biol 205, 295-302.