HoloMonitor® M4 Live Cell Analysis System

The system is very easy to use. You set up an experiment, place the cell culture vessel under the microscope, and select the experiment of interest (dose-response). The system auto-calibrates itself and collects data in real time. Sometimes external factors such as condensation on cell culture flask interfere with single-cell detection. This is visible in the images, moreover, the system flags low-quality images, which you can exclude from the data analysis. At the end of the experiments, the system generates all the figures for you (graphs, images, videos). You can then use the collected data and have them re-analyzed by the software using a different application e.g. proliferation, motility, etc. All in one, performing one experiment allows you to collect data using multiple applications. The PHI lab team are friendly, approachable and caring. The team always tried their best to help us solve issues, guide us through applications we were not familiar with or provide training where needed. At times, I needed help with the calibration of the laser, solving the accumulation of condensation on my cell culture flask. Equally, the system generates so much data that sometimes I needed help with analysis. The PHI lab team were always happy to help, responding quickly to all queries, and having video calls to navigate us through the process. The system generated reproducible cell proliferation data. I have run two proliferation assays in parallel. One is kinetic proliferation using HoloMonitor (real-time analysis) the other is tritiated thymidine incorporation proliferation assays (end-point analysis). The assays generated reproducible data. What I liked about the system is that you select how many positions within your cell culture flask the laser monitors and how often the laser monitors those positions. This is very useful if you are expecting the effect of the treatment to manifest within a short period or to study apoptosis. There is an additional “in-depth analysis” function which gives endless ways of analyzing your data. In addition to the applications that detect single-cell automatically and generate data automatically (wound-healing assay, proliferation, motility, dose-response, cell quality, etc.) with ready-to-export graphs, there is an “in-depth analysis” function. This enables you to look at cell tree plots, 48 morphological features collected for every single cell at the selected position, like roughness, and eccentricity, which you can then use to investigate the effects of the treatment on your cell morphology. The “in-depth analysis” provides infinite options to analyze your cells. This application, however, is less intuitive to use. The single-cells are detected automatically by software, but I recommend double-checking the single-cell selection manually (note: automatically selected borders can be manually adjusted here). I found this function extremely helpful when studying cytotoxic vs cytostatic cellular responses to treatments. After publishing my results, PHI lab contacted me to hear about my user experience. They were particularly interested to see where the system could do better and were very responsive to feedback. The only drawback of the system: -An in-depth analysis of 48 morphological features does not provide mean values. Let’s say you want to see the mean cell volume from three wells in your 6 well-plate. To do this, you would need to export the Excel data and analyse them yourself. -Statistical analysis is not incorporated within the software, so you have to manually export the data in Excel format and re-analyse them. All in all, I would highly recommend this product if you want to get a comprehensive understanding of your cell morphology and how it changes to stimuli (of your interest). I think it is good value for money because you only need to set up one experiment, which can be re-analysed in many ways.

Review Date: 5 Mar 2024 | Phase Holographic Imaging (PHI)

Easy to use system placed directly in the cell culture incubator; support various cell culture vessels including small flasks, 6-well plates, 24-well plates and other; definetly time and resource saving, once the experiment is started you get your ready results; possibility to upgrade and extend your analysis software

Review Date: 6 Jun 2023 | Phase Holographic Imaging (PHI)

A high value for money product. Easy to use and gives nice insights into adherent cell cultures and how they develop. The technological approach is very advanced and offers a high potential for the future. However, the device is very sensible to vibrations and generates often artifacts, so many images must be excluded manually. An advanced algorithm which can detect such artifacts and could automatically exclude them from analysis, as opposed to manually selecting the ones which should be analyzed, would be preferred to reduce user workload. Also a better algorithm for image segmentation (perhaps based on machine learning) would be highly preferred (also for later analysis based on image parameters).

Review Date: 20 Sept 2022 | Phase Holographic Imaging (PHI)

The system is very easy to use and generates high-quality, reproducible results. Dedicated data analysis offers detailed information on cell proliferation frequency, cell division duration, cell morphology and cell motility to precisely assess additive toxicity.

Review Date: 1 Aug 2022 | Phase Holographic Imaging (PHI)

The HoloMonitor machine is efficient for operating up to 96 samples simultaneously. Holomonitor can provide high-quality images and time-lapse movies. We always get professional operation instructions and data analysis suggestions from the service team.

Review Date: 2 Jul 2022 | Phase Holographic Imaging (PHI)

I am using HoloMonitor system for analyzing migration, morphology, and cell growth of cells from cell lines and primary cells, and I am very happy with the imaging system and using it a lot in my research. It is easy to use and you can get amazing data: videos/images of the cells (2D, 3D, magnifications, etc) and a range of different parameters from one single experiment. I believe it is a big advantage that it is label-free and inside the incubator, making it a more stable, "natural" milieu and less interference with the cells, and also it enables long experiments. Great microscope!

Review Date: 23 Nov 2021 | Phase Holographic Imaging (PHI)

This microscope is perfectly suited for our cell culture under flow application as well as many other types of experiments where tracking cell behaviour and motility in real-time is a must. Because it even provides morphology data in 3D we now have insights into what the cells are doing at a level which we simply could not achieve before. Highly recommended.

Review Date: 18 Nov 2021 | Phase Holographic Imaging (PHI)

The HoloMonitor M4 is a beneficial device for live-cell imaging, providing a lot of data. The device does not require large space and the software application is user-friendly, while recorded pictures look great. The good thing is that you can analyze your experiment using one software assay, and later, you can rerun the recorded data in another assay, which allows you to get the maximum from your experiment. I like it and would recommend it to all people doing a cytotoxicity assessment based on cell proliferation and optical thickness of the adherent unstained cells. Cell migration tracing is another application I like and recommend.

Review Date: 3 Nov 2021 | Phase Holographic Imaging (PHI)

I'm the principal investigator of a small research group at a public primarily undergraduate institution. Even considering our limited resources, I believe that the Holomonitor M4 is a fairly priced system and is definitely worth the investment. It has truly been and will remain a critical component of our research program. Our microscope lives directly in our cell culture incubator and allows us to see how various treatments affect our cells in real-time with three-dimensional single-cell resolution - it's amazing! The Holomonitor App Suite software is particularly powerful allowing us to quantify changes in cell morphology and movement. Importantly, I also think this instrument is great for education and my students are absolutely fascinated by the quality and beauty of the videos produced. This system is intuitive to operate and it is very effective in generating label-free long duration time-lapse videos. We dissociate complex tissues containing a mixed population of cells for culture, and with the Holomonitor M4 we are able to easily distinguish different cell types by their unique size, shape, and movement patterns. We can't wait to see what new biology we will discover with this innovative approach! I highly recommend it!

Review Date: 20 Oct 2021 | Phase Holographic Imaging (PHI)

The HoloMonitor system is easier to use than I would have expected from a product with such a range, and the software makes it a breeze to produce and reproduce, high-quality analyses of cell growth. We discovered new avenues of investigation when we could gather such detailed reports on how cells behaved in real time. For too long, in vitro cell culture has had to gather evidence in the dark. Using the HoloMonitor lets us monitor our cells as closely as we wanted to, and showing others actual footage of our results was priceless!

Review Date: 12 Oct 2021 | Phase Holographic Imaging (PHI)