The HiDef Detection™ Polymer System is a visualization system that is ready-to-use in immunohistochemical protocols. This two-step system uses an indirect method resulting in an antibody-enzyme complex that universally detects mouse and rabbit primary antibodies. The resulting chromogenic reaction can be visualized by HRP-compatible chromogens using light microscopy. It is biotin-free and eliminates non-specific staining that could result from endogenous biotin. This visualization system consists of two detection reagents and is based on the sequential application of HiDef Detection™ Amplifier (Mouse and Rabbit) followed by HiDef Detection™ HRP Polymer Detector, amplifying the detection of low expressing antigens. This detection system is compatible with both manual and open automated staining platforms.