G-LISA™ Small G-protein Activation Assays by Cytoskeleton Inc.

Manufacturer Cytoskeleton Inc.
4.3
/
5.0
  |  1 reviews
The G-LISA™ assay uses a 96-well plate coated with RBD domain of Rho-family effector proteins. The active GTP-bound form of the Rho-family protein, but not the inactive GDP-bound form, from a biological sample will bind to the plate. Bound active Rho-family protein is then detected by incubation with a specific primary antibody followed by a secondary antibody conjugated to HRP. The signal is then developed with OPD or chemiluminescence reagents. These assays are simple, fast (<3 hours), requ... Read more


G-LISA™ Small G-protein Activation Assays by Cytoskeleton Inc. product image
G-LISA™ Small G-protein Activation Assays

Cytoskeleton Inc.

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Average Rating: 4.3
1 Scientist has reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
3 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2010

  • Organization: NORTHWESTERN UNIV. SCHOOL OF MEDICINE



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
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Rating: 4.3

  • Application Area: Signaling research

"Excellent kit, works every time. But it's very expensive, so I would recommend using it only when it's important to get results fast."

Review date: 07 Jun 2012 | G-LISA™ Small G-protein Activation Assays
The G-LISA™ assay uses a 96-well plate coated with RBD domain of Rho-family effector proteins. The active GTP-bound form of the Rho-family protein, but not the inactive GDP-bound form, from a biological sample will bind to the plate. Bound active Rho-family protein is then detected by incubation with a specific primary antibody followed by a secondary antibody conjugated to HRP. The signal is then developed with OPD or chemiluminescence reagents. These assays are simple, fast (<3 hours), require only small amounts of sample (5-25 µg cell protein), yield quantitative and accurate data and are suitable for high thoughput screens.

Product Overview

G-LISA™ Small G-protein Activation Assays by Cytoskeleton Inc. product image

G-LISA™ Small G-protein Activation Assays

Manufacturer Cytoskeleton Inc.

4.3 / 5.0 | 1 reviews