Status:
ReviewerMember since: 2019
Organization: Hospital Clínic
Although by now it is not the gold standard, it will be in the near future.
Application Area: Measuring Protein-Binding Affinities
"An easy to use equipment that allows to easily define the binding of proteins. Results are reproducible and comparable (not exact) to Biacore."
The Fluidity One-W’s unique capabilities allow you to study protein complexes and their formation in crude biological backgrounds such as lysates or blood plasma.
Critically measurement of protein size by Fluidity One-W helps determine the identity of your complex and means you can control for off-target binding and false-positive measurements.
Binding is identified by simple size change from free to bound species — because there is no surface attachment this means you can study proteins in near-native conditions.
Binding affinity (K D ) and stoichiometry of binding are quickly and easily determined. All measurements are performed fully in solution, with no immobilization to a surface.
The award-winning microfluidic diffusional sizing (MDS) technology allows highly sensitive analysis, even at low concentrations.