Status:
ReviewerMember since: 2021
Organization: Intravacc
Unsurpassed quality and support.
Application Area: Analysis of intermediate and final vaccine products like Viruses, OMV's, proteins, polysaccharides a
"The quality is of the highest standard. The support we get is unsurpassed, and fails to compare to any other company. We now see a welcomed trend towards GMP/CFR21 Part11 compliant, which will help us to incorporate the Wyatt instrumentation in our production processes. Especially for our outer membrane vesicles and conjugate vaccine development there is no other instrumentation that could help us to develop such vaccines to such high standard."
Status:
ReviewerMember since: 2020
Organization: STEMCELL Technologies
Great instrument that has significantly improved our research capabilities!
Application Area:Characterization of antibodies, nanoparticles and extracellular vesicles
"We've had the Eclipse AF4 flow field flow fractionation system in our research and development lab for over a year now. The system itself is very easy to use, especially if you have a basic understanding of HPLC and/or SEC. We have been able to obtain successful separation of samples containing both digested and undigested antibodies, and isolation of nanoparticles from multiple supernatant components. Wyatt customer service is great - every time we have run into an issue, we have gotten a response from support in less than 24 hours and they have always been able to solve our problem. The instrument is very expensive, but it is highly versatile and has become a key piece of instrumentation in our lab."
Status:
ReviewerMember since: 2020
Organization: Georgia Institute of Technology
It is our view that the Wyatt Eclipse AF4 will replace GPC as the industry standard.
Application Area:Molar mass, size and shape information for water soluble polymers, proteins and particles
"Field flow fractionation is a very difficult technique to master but the Wyatt Eclipse AF4 unit with included Scout predictive software, in addition to their 24/7 customer service, made method development and analyte characterization very achievable and enjoyable. Using one single instrument we have managed to separate and characterize polyelectrolytes with small radii from several nm up to hundreds of nm long rod like cellulose nanocrystals. The versatility and capability of this instrument is incredible and Wyatt's field reps must be credited for helping us realize the full potential of what AF4 can do."
Status:
ReviewerMember since: 2020
Organization: Eawag
Wyatt Europe is the best partner for all service. Interesting webinars and exceptional customer service.
Application Area:Analyzing NP-particle interactions with organic colloids in environmental systems
"With Wyatt Europe, I have a really good partner in after sales and application development/method development. The system is very flexible and with the service contract I always can count on immediate remote-help. Although, I am not very happy with the dictated combination of hardware and software developments meaning that I am somewhat dependend on expensive software-changes for optimal use of the hardware, and respective bug-fixes. As a university research institute we don't have the money to change working hardware, like in industrial settings. Wyatt US only provides online help if the instrumentation is not older than 10years, which means the possibility to satisfy customers like us is only possible by making a lot of effort. The free webinars offered to us during COVID19 were more than helpful, including the user seminars in the last weeks."
Status:
ReviewerMember since: 2019
Organization: Zentriforce Pharma
Nice and easy to use AF4 system.
Application Area:ATMP, Peptides and Antibodies
"Nice and easy to use AF4 system. For an AF4 system it is amazingly robust and can be applied to a wide range of applications. The option to use a frit-inlet channel is useful to test for focus stress induced changes of your material."
Status:
ReviewerMember since: 2019
Organization: IBT-VAST
Great system, must-have for high IF paper.
Application Area:Exosome fractionation
"The system works well for the purpose of study which is separation of exosome based on size. Although setup and optimization of instrument are quite complicated, the support staff are well-trained and professional. I usually get replies to emails email within 1-3 working days."
Status:
ReviewerMember since: 2019
Organization: Seqirus Inc.
AF4 has great potential to fractionationate without the adverse effects of LC/SEC.
Application Area:Influenza vaccines, proteins, lipid nanoparticles,
"The instrument is designed well for ease of use in batch or being modular for online mode. However, the software needs to be well integrated so that it is a more cohesive software package where all of its capabilities are centralized into one package. As a result the software has a steep learning curve. Also, the AF4 channel design can definitly be improved with torqued latches so that assembly can be done more simplistically by flipping latches instead of manually by hand with a torque wrench."
Status:
ReviewerMember since: 2017
Organization: Leidos Biomedical Inc.
Easy to use
Application Area:Nanoparticle characterization
"We used Wyatt Eclipse AF4 coupled with online MALS and DLS for liposome, nanoparticle, and other formulation characterization. It is easy to use, and is able to produce high quality results."
Status:
ReviewerMember since: 2017
Organization: 3M (Pace Analytical)
This instrument has proved invaluable for the analysis of very difficult samples.
Application Area:polymers, nanoparticles
"This system is able to separate ultra high molecular weight polymers as well as highly branched polymers. Additionally, we are able to determine the radius of different sized nanoparticles and colloids."
Status:
ReviewerMember since: 2016
Organization: Lund University
I had good results
Application Area:macromolecules
"Asymmetrical flow field-flow fractionation when connected to suitable detector such as multi-angle light scattering (MALS) has shown to be a great technique for the analysis molar mass and root mean square of many food macromolecules. The results are reproducible and with good quality"
The Eclipse™ solves many difficult separation, fractionation and sizing tasks for macromolecules, nanoparticles and colloids by field-flow fractionation (FFF). Combined with Wyatt’s DAWN® or miniDAWN® multi-angle light scattering (MALS) detector and Optilab® refractive index detector, the Eclipse offers a uniquely versatile FFF-MALS solution providing characterization of absolute molar mass, size, particle concentration, conformation and encapsulation.
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Manufacturer Waters | Wyatt Technology | Available Worldwide
4.7 / 5.0 | 10 reviews