DNMT Activity / Inhibition Assay by Active Motif

Manufacturer Active Motif  |   Model: 55006
Highly sensitive and rapid microplate-based assay to simplify the measurement of DNA methyltransferase activity


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DNMT Activity / Inhibition Assay

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The DNMT Activity / Inhibition Assay is a non-radioactive assay to measure the activity or inhibition of DNA methyltransferases.

The sensitive ELISA-based method utililzes the high affinity binding of methyl CpG binding domain (MBD) protein towards methylated DNA in order to detect DNA methyltransferase activity on the provided CpG-enriched DNA substrate. The assay is performed in a 96-stripwell plate, enabling low or high throughput screening that is easily quantified by spectrophotometry.The DNMT Activity / Inhibition Assay provides everything needed to study DNA methyltransferase activity from recombinant DNMT enzymes (DNMT1, DNMT3a & DNMT3b) or nuclear extract samples. A CpG Methyltransferase is included as a positive control.