CellDrop™ Automated Cell Counters

Trypan Blue is widely used in cell culture labs for selectively staining dead cells and tissues. However, some sample types can present challenges for accurately assessing cell counts and viability with Trypan Blue. For these sample types, a fluorescence assay such as Acridine Orange / Propidium Iodide (AO/PI) is a simple and highly accurate alternative. Learn about each viability method and the situations where fluorescence is advantageous in this free eBook.

Isolating clean, intact nuclei is vital for the success of single-nucleus workflows such as RNA sequencing (RNA-seq) and Assay for Transposase-Accessible Chromatin with sequencing (ATAC-seq). High-quality, debris-free samples are essential for accurate library preparation and downstream analyses, as debris can hinder precise nuclei counting. Traditional manual counting methods, while reliable, are time-consuming and prone to human error, especially with large sample volumes.

This eBook explores automated nuclei counting and quality control using the DeNovix CellDrop™ Automated Cell Counter. With features like DirectPipette™ technology that eliminates costly consumables, advanced fluorescence applications, and user-friendly software, researchers can improve accuracy, efficiency, and consistency in sample preparation. Discover practical tips to streamline workflows, minimize variability, and enhance experimental outcomes.

The requirement for ever-greater focus and precision has driven the development of improved single-cell sequencing analysis technologies. Techniques such as RNA sequencing and ATAC sequencing require intact single nuclei, so the ability to isolate individual nuclei from sample tissue is therefore critical to the success of these sequencing approaches. Isolating nuclei that are non-clustered and free from debris is critical both to successful library preparation and the ability to count nuclei accurately.

To help you achieve isolation of nuclei for your single-cell genomics workflows, this expert guide outlines:

• Automated counting of isolated nuclei
• Best practices for cell counting
• Improving cell counting accuracy

It will also cover two case studies – frozen brain tissue and plant protoplasts – including technical notes.

Download your free copy to learn more about the key features and applications of the CellDrop™ FL Automated Cell Counter from DeNovix as well as how the DeNovix DirectPipette™ technology is designed to eliminate plastic slides and cumbersome hemocytometers from routine cell counting.

In this application note, DeNovix introduces the CellDrop™ automated cell counter, which offers cell counting without the use of plastic slides. The CellDrop features dual fluorescence and brightfield optics, a variable height sample chamber, and powerful analysis software. It can quantify and qualify the viability of primary cell samples, such as peripheral blood mononuclear cells (PBMCs) using viability dyes to count whole blood samples. It is also optimized for accurate cell counts and viability across a wide range of sample types, including those with significant debris or minimal debris. Recommended viability assays are provided for common sample types.

In this application note, DeNovix shows how to count isolated nuclei on the CellDrop™ Automated Cell Counter, advancing quality control methods for single nuclei sample preparation.

Nuclei isolated from tissue samples such as a brain have high levels of debris, which in turn can affect downstream single-cell sequencing workflows and result in low-quality libraries or failed sequencing experiments.

In this application note, DeNovix demonstrates how dual fluorescence counting using the CellDrop™ FL Automated Cell Counter can clearly distinguish nuclei, unlysed intact cells, and debris by acridine orange / propidium iodide (AO/PI) counting. Download the full note below to learn how this protocol was used to accurately count isolated nuclei from mouse brain.

In this technical note, DeNovix gives a detailed description of how to optimize the AOPI or Custom Methods Applications for counting protoplasts on the DeNovix CellDrop™ FL Automated Cell Counter in conjunction with fluorescein diacetate (FDA) and propidium iodide(PI) fluorescent dyes.

Cell counting and viability measurements are important for a wide range of research applications across many diverse sample types. This application note describes seven top tips from the to ensure accurate and reproducible results when counting cells manually or using automated systems such as the CellDrop™ Automated Cell Counter.

Primary cell samples are challenging to accurately quantify and qualify viability using traditional cell counting methods. In addition to the inherent variability in counting between human operators, PBMCs are difficult to discern from red blood cells (RBCs). This application note describes a robust method for PBMC enumeration on the automated CellDrop FL cell counter.

Take the hassle out of preparing sample slides for cell counting with the CellDrop™ Automated Cell Counter. This user-friendly device is designed to load cell suspensions directly onto durable, scratch-proof sapphire surfaces, eliminating the need for disposable slides. Watch in this video demonstration as it delivers a live HD view of cells and produces an accurate brightfield cell count in less than three seconds.

Discover how the CellDrop™ automated cell counter overcomes the challenges of counting organoids, tumorspheres, and spheroids. See how advanced machine learning enhances accuracy, and watch a live demo of organoid counting in action.

In this on-demand webinar, DeNovix Application Scientist Ben Capozzolli discusses a method for accurate and reproducible nuclei counts and shares tips for preparing nuceli samples before single-cell sequencing workflows.

In this video, DeNovix application scientist, Ben Capozzoli, demonstrates the process of counting single nuclei suspensions on the CellDrop™ Automated Cell Counter. He emphasizes the significance of isolating nuclei for various workflows like ATAC-sequencing and RNA-sequencing. Having precise nuclei counts and knowledge of debris content is crucial for successful downstream applications. The CellDrop excels in accurately determining nuclei counts and extraction efficiency scores, irrespective of debris presence.

In this video, DeNovix describes the positive impact of the CellDrop™ Automated Cell Counter on environmental sustainability by reducing the use of one-use plastic slides.

Trypan Blue is widely used in cell culture labs for selectively staining dead cells and tissues. The use of Trypan Blue dye can present challenges for accurately assessing cell counts and viability in some sample types. For these samples, a fluorescence assay such as Acridine Orange / Propidium Iodide (AO/PI) is a simple and highly accurate alternative.

Using the CellDrop Automated Cell Counter, DeNovix Scientific Director Dan Schieffer demonstrates both brightfield and fluorescence methods of cell counting and viability assessment.

In this video, DeNovix introduces the CellDrop Cell Counters that enable users to count cells without slides, eliminating the need for plastic slides. Applications include brightfield cell counting, fluorescence cell counting and viability.

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DeNovix has released a new model in the CellDrop Automated Cell Counter range, with enhanced performance and new cell counting applications

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