Automated counting of isolated nuclei: Transform your single-cell genomics workflow

24 Jul 2023
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The requirement for ever-greater focus and precision has driven the development of improved single-cell sequencing analysis technologies. Techniques such as RNA sequencing and ATAC sequencing require intact single nuclei, so the ability to isolate individual nuclei from sample tissue is therefore critical to the success of these sequencing approaches. Isolating nuclei that are non-clustered and free from debris is critical both to successful library preparation and the ability to count nuclei accurately.

To help you achieve isolation of nuclei for your single-cell genomics workflows, this expert guide outlines:

  • Automated counting of isolated nuclei
  • Best practices for cell counting
  • Improving cell counting accuracy

It will also cover two case studies – frozen brain tissue and plant protoplasts – including technical notes.

Download your free copy to learn more about the key features and applications of the CellDrop™ FL Automated Cell Counter from DeNovix as well as how the DeNovix DirectPipette™ technology is designed to eliminate plastic slides and cumbersome hemocytometers from routine cell counting.

CellDrop™ Automated Cell Counters

DeNovix

Reduce consumable costs and improve lab sustainability with patented DirectPipette™ technology, or use slide mode for ultimate measurement flexibility. The CellDrop features dual fluorescence and brightfield optics, variable height sample chamber to make cell counting fast, simple, and reproducible.

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.Single Cell Genomics
Automated counting of isolated nuclei: Transform your single-cell genomics workflow