Regardless of next-generation sequencing (NGS) platform, universal and index adapter sequences are required for the proper assembly of sample fragments. Adapters – especially index adapters since they contain the MID (multiplex identifier) or barcode – containing too high a proportion of truncated sequences (unacceptably low purity) or too high a proportion of other adapter sequences (excessive cross contamination) can lead to compromised sequence read integrity (excessive adapter dimerization too) and improper sequence assembly, respectively, during multiplexing experiments. These types of problems are undetectable until the data analysis stage, which make them costly in terms of time and money. Therefore, the production process used for the adapter sequences is critical for a successful sequencing run. Our custom Next-Gen Sequencing Oligos (NGSO) are manufactured under rigorous conditions to ensure suitable purity and low cross contamination.
Manufacturer The Life Science business of Merck KGaA, Darmstadt, Germany, operates as MilliporeSigma in the U.S. and Canada | Available Worldwide