Member since: 2019
Lasted 13 months before it died - conveniently just out of warranty.
Application Area: Cell counting
"It was easy to use except that sometimes when powered off it would freeze starting up and need to be restarted a couple of times. Then we turned it off over Christmas and it wouldn't start back up again at all. We've had it for exactly 13 months, so of course it's no longer covered by warranty. A very expensive piece of equipment to only last 1 year and be completely useless due to what appears to be the result of buggy software. Would not recommend."
Member since: 2017
Very useful for our sn and scRNAseq applications.
Application Area:Cell or nuclei counting
"Use this product with the EVOS GFP light cube for cell and nuclei staining, works great and is very useful for our needs."
Member since: 2017
Organization: Altius Institute for Biomedical Sciences
A must for any lab that does considerable cell culture work.
Application Area:Counting cells
"Fantastic, time-saving instrument. Incredibly simple to use. Struggles a little with smaller/primary hematopoietic cells. Fluorescence detection more suited to very highly expressing cells"
Member since: 2016
Super easy to use, even for a novice
Application Area:Cell counting for single cell library prep
"Getting accurate cell counts for single cell library prep is essential. Having never performed cell counting before, I was worried about variability and accuracy. This little instrument is incredibly easy to use with no prior experience and appears to be very accurate for our purposes."
The new Countess™ II FL Automated Cell Counter is a benchtop assay platform equipped with state-of-the-art optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. With three-channel flexibility—brightfield and two optional fluorescence channels—researchers can count cells, monitor fluorescent protein expression, evaluate apoptosis, and measure cell viability.
• Accurate—use the Countess II FL Automated Cell Counter to eliminate the subjectivity of manual cell counting and user-to-user variability
• Flexible—the Countess II FL Automated Cell Counter is able to accept up to two EVOS® light cubes that are user-selectable from over twenty fluorescence channels
• Fast—the Countess II FL Automated Cell Counter provides a total cell count, reports viability, and measures average cell size in as little as 10 seconds. Counting speed is identical when using fluorescence channels.
• Convenient—the Countess II FL Automated Cell Counter requires no cleaning or routine maintenance. A capacitive touchscreen and simple user interface provides quick startup and requires minimal training.
Learn more about our automated cell counters >
The Countess II FL Automated Cell Counter comes with:
• Countess USB drive for storing and transferring data to your computer
• Disposable slide holder
• Reusable slide holder
• Light cube removal tool
• Power cord and 4 plug adaptors to accommodate most countries
• Quick reference card (QRC)
• A free box of 50 disposable slides
The Countess II FL Automated Cell Counter works in 3 simple steps:
1. Mix 10 µL of sample with 10 µL of trypan blue and pipet into a disposable Countess chamber slide.
2. Insert the slide into the instrument.
3. Press the "Count" button.
Get accurate cell counts
The Countess II FL Automated Cell Counter uses trypan blue staining combined with an auto-focus mechanism and sophisticated image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 x 10e4 to 1 x 10e7 cells/mL (the optimal range is 1 x 10e5 to 4 x 10e6 cells/mL), which is broader than that of a hemocytometer. The optimal cell size for accurate viability assessment is 7–60 µm, while particles as small as 4 µm can be successfully counted.
Simple operation with fluorescent application versatility
Operated using an intuitive user interface, automated cell counting with the Countess II FL instrument gives you the data you need to prepare your sample for the next passage or experiment, and allows you to multiplex with a variety of fluorescent dyes and proteins excited via EVOS® Light Cubes.
Transfer data and images to your PC
Using the USB port on the Countess II FL Automated Cell Counter, you can save your cell count results and images, and then transfer them to your PC. (For your convenience, a USB drive is supplied with the Countess II FL instrument).