Corning® Matrigel® hESC-Qualified Matrix, LDEV-free, 5 mL
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.Quality Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing Extensive…
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Good but difficult growth.
Intestinal organoids
It's difficult to know how much you have to use or if it affects the growth if you dilute it.
Review Date: 3 Jun 2020 | Corning Life Sciences
Best for Non-MEFS iPSC culturing.
iPSC culture
For coating of plates for iPSC. Need to make sure matrigel is always on ice, otherwise it will seize up.
Review Date: 23 Mar 2018 | Corning Life Sciences
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Quality
- Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
- Extensive PCR testing is performed to screen for a number of pathogens
- Tested and found negative for bacteria, fungi, and mycoplasma
- Protein concentrations determined by Lowry method
- Endotoxin units measured by Limulus Amoebocyte Lysate assay
- Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
- Biological activity is determined for each lot using a neurite outgrowth assay
Source
Engelbreth-Holm-Swarm (EHS) mouse tumor
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws.
For Customers in California only: WARNING Please refer to Safety Data Sheet (SDS) Section 15 for more information regarding California Proposition 65.
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In this application note, the formation of gastrointestinal organoids derived from human induced pluripotent stem cells (hiPSC) is demonstrated — while using Corning® 96-well spheroid microplates, in combination with Corning Matrigel® matrix. This results in a single intestinal organoid in each well. Differentiation into definitive endoderm and intestinal lineage is confirmed by flow cytometry and immunostaining methods.
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In this application note, a novel high-throughput gene expression analysis technology — the nanoString nCounter® platform — is used to characterize gene expression of airway organoids generated from healthy, and asthmatic, primary bronchial cells. The nanoString nCounter PlexSet assay provides a simple and cost-effective solution for multiplex sample analysis of up to 96 custom probes across 96 samples per run. The assay uses whole cell lysate, without the need for RNA purification, and was simple to incorporate into the airway organoid workflow. The end result is high-throughput gene expression data without amplification, cDNAconversion or library prep.
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A Novel Method for Generating Single Intestinal Organoids for High-Throughput Screening
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