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High-Throughput Gene Expression Analysis of 3D Airway Organoids

Corning Life Sciences
25 Mar 2019

In this application note, a novel high-throughput gene expression analysis technology — the nanoString nCounter® platform — is used to characterize gene expression of airway organoids generated from healthy, and asthmatic, primary bronchial cells. The nanoString nCounter PlexSet assay provides a simple and cost-effective solution for multiplex sample analysis of up to 96 custom probes across 96 samples per run. The assay uses whole cell lysate, without the need for RNA purification, and was simple to incorporate into the airway organoid workflow. The end result is high-throughput gene expression data without amplification, cDNAconversion or library prep.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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High-Throughput Gene Expression Analysis of 3D Airway Organoids