Bovine serum albumin (BSA) is used extensively as a carrier protein to dilute antibodies and as a general protein blocking agent in immunoassays and immunodetection protocols.
Our IgG-free BSA, which is also protease-free, should alleviate many of the problems associated with using BSA.
Many preparations of BSA, even some of the highest purity grades, contain IgG that may become an antigen for cross-reacting secondary antibodies. This is particularly common when using anti-bovine IgG, anti-goat IgG (with the exception of bovine anti-goat IgG), and anti-sheep IgG, but may occur with other antibodies that cross-react with bovine IgG as well. The result of these interactions may be loss of desired antibody activity, loss of antibody stability, and/or increased background. Background may derive from sticky soluble immune complexes or from contaminating bovine IgG sticking non-specifically and attracting cross-reacting labeled secondary antibodies. Even small amounts of contaminating IgG may create these problems due to the use of high concentrations of BSA in many protocols.