Bioruptor® Plus
The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. An excellent device for shearing chromatin or DNA, cell and tissue disruption and many other applications. Diagenode’s Bioruptor® Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and othe…
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Highly effective
Protein aggregation and seeding
Easy to use, works well if you want to purify amyloids from tissue samples.
Review Date: 25 Sept 2022 | Diagenode
So far it is good.
Sonication, I POND, ChIP
We just bought the Bioruptor, so far it is good.
Review Date: 30 Apr 2019 | Diagenode
Reproducible results every time
Sonication for ChIP-seq-eq
Very easy to use, no worries about the heating, high throughput.
Review Date: 29 Nov 2016 | Diagenode
Great Instrument and considered a must have in our lab.
Extraction of protein & RNA/DNA from small volume tissue samples for downstream analysis.
Easy setup and straightforward to operate. Great technology in that it keeps your samples at low temps during sonication/homogenization of tissues and is high efficiency. Process is contained within each tube so there's no risk of cross-contamination.
Review Date: 28 Jun 2016 | Diagenode
A must have when performing ChIP
Tissue Homogenization for ChIP
Excellent instrument for DNA shearing from tissue. Had attempted using a sonicator which destroyed my DNA and protein. The Bioruptor consistently sheared my DNA to an appropriate size and did not disrupt the protein.
Review Date: 18 Dec 2015 | Diagenode
The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. An excellent device for shearing chromatin or DNA, cell and tissue disruption and many other applications.
Diagenode’s Bioruptor® Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. The Bioruptor® Sonication System uses a sonication bath to generate indirect sonication waves, which emanate from an ultrasound element below the water tank. Because the system is gentler than other sonicators, the Bioruptor® produces better and more consistent results than with harsher sonication methods. Up to 12 closed tubes can be sonicated in parallel and the continuous rotation of tubes allows even distribution of the energy for efficient sonication. The Bioruptor® enables automation of sonication, guaranteeing higher reproducibility of results.
Features:
- Digital timer
- Metal soundproof box
- Overheat shutdown protection (this feature is not available in the US)
- Machine lifetime control
Applications:
- Chromatin Shearing
- DNA Shearing
- Cell & Tissue disruption
Evaluation of Bioruptor in Shotgun Library Preparation for Massively Parallel Sequencing on Illumina Genome Analyzer II
The Illumina Genome Analyzer II (GA-II) performs massively parallel short-read DNA sequencing, with the potential to generate up to 10 gigabases in a single run. The technology provides researchers with a significantly cheaper alternative to conventional sequencing by capillary electrophoresis. A consistent and reliable method of DNA fragmentation is essential in Illumina library construction. This application note evaluates and compares two types of fragmentation methods: nebulization and shearing using the Bioruptor.
Bioruptor® NGS: Unbiased DNA shearing for Next-Generation Sequencing
This application note describes the use of the Bioruptor® NGS to prepare the samples in the sequencing of two bacterial genomes with dissimilar GC characteristics - Escherichia coli and Staphylococcus epidermis.
RNA Extraction from Tissue Using Bioruptor® (Standard/Plus) and RNA Extraction Kit from Diagenode
Isolation of intact RNA is essential for many techniques used in gene expression analysis. Diagenode’s Bioruptor® Sonicator uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues in one step and Diagenode’s RNA extraction reagent (included in the RNA extraction kit) is used as sonication medium to maintain the integrity of RNA while disrupting cells and dissolving cell components. Bioruptor® together with RNA extraction reagent offer unique benefits for tissue disruption and homogenization. The protocol is fast, efficient, reproducible and minimizes contamination through non-contact.
Chromatin Preparation From Tissues for Chromatin Immunoprecipitation Using Diagenode’s Chromatin Shearing Optimization Kit - Low SDS and Bioruptor®
This application note describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.
Bacterial Cell Disruption Using Bioruptor® Plus
Disruption of bacterial cells is required for releasing biological molecules from within the cells for various biological applications. Ultrasound technology has been widely used for such cell lysis applications. Ultrasonic disruption is based on cavitation, the creation of cavities in cell suspension. These cavities or small bubbles of dissolved gases or vapors arise from the alteration of pressure in liquids. The bubbles are capable of resonance vibration and produce vigorous eddying or microstreaming leading to mechanical stress that effectively lyses cells. Diagenode’s Bioruptor® sonication device uses state-of-the-art ultrasound technology to disrupt cells for biological, chemical, pharmaceutical, and industrial applications. The Bioruptor® causes no contamination between samples, gentle processing and has a multiplexing capability. This application note describes a simple, effective and rapid protocol for lysis of bacteria using the Bioruptor®.
High Quality ChIP-seq Experiments with Diagenode Automation Instruments
Watch this video to learn how Diagenode, the leader in the field of epigenetics, provides full solutions for your chromatin immunoprecipitation and DNA methylation research with its wide range of automation instruments.
