Bacterial Cell Disruption Using Bioruptor® Plus
19 Oct 2014Disruption of bacterial cells is required for releasing biological molecules from within the cells for various biological applications. Ultrasound technology has been widely used for such cell lysis applications. Ultrasonic disruption is based on cavitation, the creation of cavities in cell suspension. These cavities or small bubbles of dissolved gases or vapors arise from the alteration of pressure in liquids. The bubbles are capable of resonance vibration and produce vigorous eddying or microstreaming leading to mechanical stress that effectively lyses cells. Diagenode’s Bioruptor® sonication device uses state-of-the-art ultrasound technology to disrupt cells for biological, chemical, pharmaceutical, and industrial applications. The Bioruptor® causes no contamination between samples, gentle processing and has a multiplexing capability. This application note describes a simple, effective and rapid protocol for lysis of bacteria using the Bioruptor®.
Related Products
Request Quote for All Products
Bioruptor® Plus
DiagenodeThe Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. An excellent device for shearing chromatin or DNA, cell and tissue disruption and many other applications. Diagenode’s Bioruptor® Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. The Bioruptor® Sonication System uses a sonication bath to generate indirect sonication waves, which emanate from an ultrasound element below the water tank. Because the system is gentler than other sonicators, the Bioruptor® produces better and more consistent results than with harsher sonication methods. Up to 12 closed tubes can be sonicated in parallel and the continuous rotation of tubes allows even distribution of the energy for efficient sonication. The Bioruptor® enables automation of sonication, guaranteeing higher reproducibility of results. Features: Digital timer Metal soundproof box Overheat shutdown protection (this feature is not available in the US) Machine lifetime control Applications: Chromatin Shearing DNA Shearing Cell & Tissue disruption