Axcend Focus LC®

With high performance liquid chromatography (HPLC) being one of the most widely used analytical techniques in the world, it is crucial to feel confident in its ability to produce repeatable separations.

Explore the capillary scale HPLC – Axcend Focus LC®. Learn about how well it performed in both single- and multi-day repeatability studies and how its ability to achieve this performance with significantly lower solvent and sample volumes highlights its potential as a greener and more economical alternative to traditional HPLC systems.

Axcend introduces its line of eco-friendly, compact, and portable HPLC instruments and accessories, engineered to deliver excellent performance and repeatability in a sustainable, flexible format. Including, an overview of its compact and portable autosampler for the Axcend Focus LC®.

Therapeutic Drugs of Abuse testing for critical point-of-care treatment, such as in drug treatment facilities or pain clinics, has traditionally been arduous, expensive and time consuming, requiring a third-party laboratory for analysis. Due to these factors, on-site liquid chromatography (LC) analysis at the local clinic is a requisite for timely evaluation and effective treatment. A point-of-care LC system must be easy to use, self-contained, safe for the user, hand portable, and compact to be effective. In this application note, Axcend shows how the innovative Axcend Focus LC® (AFLC) is well suited for point-of-care Drugs of Abuse testing.

The Axcend Focus LC® High-Performance Liquid Chromatography system (HPLC) delivers repeatable analyses of Active Pharmaceutical Ingredient (API) dissolution. In this application note, an Extra Strength Excedrin® analgesic tablet was dissolved, without stirring, in a beaker containing 200 mL of media [97% water; 3% acetonitrile (ACN); and <0.1% trifluroacetic acid (TFA)]. With samples acquired approximately every 12 minutes, the three APIs (acetaminophen, aspirin, and caffeine) were well separated at 4.5 min/run with an average retention time of 2.75 min, +/- 0.05 min RSD (relative standard deviation). Additionally, only 513 µL (0.51 mL) of organic and aqueous solvents were consumed during the 12-hour experiment, resulting in dramatically lower costs for solvents and waste disposal.

In this application note, the Axcend Focus LC® was used to separate polycyclic aromatic hydrocarbons (PAHs) in ~12 and ~15 minutes, respectively, with separate methods on short (10 cm x 150 µm i.d.) capillary columns packed with 1.7 µm monomeric and 3.0 diameter polymeric C18 particles. As a lightweight, hand-portable, AC- or DC- powered high-performance liquid chromatography system that utilizes capillary columns and on-column UV-absorption detection, the Axcend Focus LC is uniquely suited for both in-the-field or laboratory-based HPLC detection, separation and analysis of a variety of target chemicals, including products and by-products of fossil fuels, pollutants, and agrichemicals, to name a few.

In this application note, Axcend demonstrates the use of hand-portable capillary HPLC by the Axcend Focus LC for intact protein analysis. Specifically, the separation of five test proteins in the range of 5.8 to 45 kDa is shown using reversed phase (RP) conditions. Other protein samples can be analyzed using the Axcend Focus LC merely by adapting established conventional high performance LC methods to the capillary column format.

February 27, 2025 - 16:00 GMT / 17:00 CET / 11:00 EST / 08:00 PST

Compact and portable high-performance liquid chromatography (HPLC) systems are revolutionizing applications such as reaction monitoring, pharmaceutical testing, and quality control by providing high-performance separation capabilities in a smaller footprint. As laboratories strive to enhance efficiency, reduce costs, and improve sustainability, these advanced systems are transforming analytical workflows across various industries.

Join us for an exclusive webinar to discover the latest advancements in compact HPLC technology with Axcend, hearing example use cases from leading pharmaceutical companies. Learn how Axcend's innovative HPLC methods are revolutionizing analytical chemistry by enhancing efficiency, lowering operational costs, and delivering reliable results in a wide range of laboratory settings.

Key learning objectives:

• Explore case studies and examples from leading pharmaceutical companies and other industries, demonstrating the benefits of compact HPLC
• Understand the practical applications of advancements in compact HPLC, including reaction monitoring, pharmaceutical testing, and use in various production settings
• Discover how small footprint HPLC systems can contribute to efficiency gains, including solvent usage reduction and waste disposal savings
• Learn about the compatibility of the Axcend HPLC with existing lab infrastructure, supporting capillary columns and integrating with ChemStation and OpenLabs

Who should attend?

Chemists, analytical scientists, analytical/quality engineers, QA/QC professionals, pharma QC professionals, HPLC users, purchasers, lab managers, and technicians

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Tuesday, May 27, 2025 at 16:00 BST / 17:00 CEST / 11:00 EDT / 08:00 PDT

Capillary scale liquid chromatography (LC) offers separations comparable to the more traditional analytical scale at a fraction of the solvent consumption. In this webinar, learn how your lab can benefit from a compact capillary scale LC instrument that can be hand-carried anywhere and used on-the-spot.

In order to be considered as a viable replacement for larger scale systems, a system must demonstrate its robustness and repeatability. Key repeatability metrics will be discussed, including interday and intraday repeatability, and injection carryover. Discover how these metrics provide valuable insights to those looking to integrate capillary scale separations into their workflows.

Key learning objectives

• See how capillary scale chromatography can help your lab save solvent and sample
• Learn about compact capillary scale instrumentation
• View methods for the quantification of system repeatability and discover how the Axcend Focus LC® performs

Who should attend?

Chemists, analytical researchers, lab directors and managers, VPs and directors of QC/QA, lab owners, and academics.

Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

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