Member since: 2019
Organization: Department of Biology, UMASS Amherst.
Simple product that is a massive time saver when cloning steps require recovery.
Application Area: Concentration of recovered DNA fragments
"These devices can save days of re-making DNA fragments when the concentration of a recovered fragment is initially too low to use in a subsequent reaction. Neat units that fit into a standard microfuge and can shrink down 4-500ul of DNA solution (or protein or other solutions) reliably into 20-25ul volume with very little loss of DNA (smaller pieces like primers do have a reduced yield). Half an hour with one of these spin columns gives around a 15-25-fold concentration of the fragment, can produce optimal fragment concentration, and allow you to skip onto the next step of the process. Simple but extremely useful."
Member since: 2019
Organization: Greenlight Biosciences
Fast, efficient spinning devices that deliver consistent results!
Application Area:Sample preparation
"These centrifugal devices are originally meant to be used for concentrating and/or desalting macromolecules [and they work very well, yielding high, consistent recoveries]. I, however, have been using them for several "off-label" passing applications and found them invaluable! Some examples: separating polymers from extended-release pharmaceutical formulations of small-molecule type drugs prior to instrumental analysis [a great alternative to SPE], measuring in vitro-release of encapsulated drugs in liposomal formulations [a faster and more representative alternative to SPE] , separating shorter length degradation products generated during stability studies of macromolecules. They are available in a wide range of volumes and nominal MW cut-offs. "
Member since: 2020
Organization: North-West University
Application Area:Isolate small metabolites from biofluids
"Great results that are repeatable. Good service from Merck."
Member since: 2006
Organization: University of Vermont
Very useful and efficient but quite costly.
Application Area:Purification of growth factors
"Amicon Centrifugal Filter devices from Millipore are very efficient and reliable"
Member since: 2015
Organization: university of cambridge
"This is a very reliable product from Amicon. It is very helpful in dealing with complex samples and is very easy to use. It has a reasonable pricing and fast purification."
Member since: 2015
Organization: The University of Texas at Austin
Application Area:Protein purification
"This product worked well in efficiently purifying my protein. I highly recommend it."
Member since: 2014
Organization: Leibniz Institute of Plant Biochemistry- Molecular Signal Processing
Application Area:Protein Purification
"Amicon Ultra concentrator filter is really fast and amazing. As they have, even for smaller volumes, reduced the loss of protein in the sample just by sticking it on to its wall. Also they are designed in such a way that it’s very easy to use. It looks like an Eppendorf tube! I will recommend this for others for using it."
Member since: 2012
Application Area:Protein Purification And Concentration
"Amicon® Ultra-0.5 Centrifugal Filter Units are useful for protein purification and concentration. This filter device can remove proteins bellow 3 kDa. This filter device can be used to concentrate a pure protein with a molecular mass above 3 kDa. The recommended velocity to spin this device is 5000-7000 RPM, however when the filter is used for long time it may be useful to decrease this speed up to 3000 RPM as there is Always chance of loss of proteins after using it for a long time. The second more important thing is to fill this device up to a maximum of 0.5 ml as there is a chance of filter loss above this volume. It is suitable for small scale protein concentration and purification. I recommend it to use for protein separation and concentration and to store this device either in 20% Ethanol or 0.02% Na-azide solution after use."