Amicon Ultra-0.5 mL Centrifugal Filters for Protein Purification and Concentration
• Concentrates 500 µL down to 15 µL• Concentration factor 25 x to 30 x• High sample recovery (greater than 90% of dilute starting solution)• Fast processing time - 10 to 30 minutes• Consistent recoveries thanks to the reverse spin capability• Reverse spin capability provides consistent recoveries
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It's make my work easy
Food and Pharma
Very easy to use and recommend other the same
Review Date: 28 Jun 2023 | Merck
Nice filters for buffer exchange and concentration
Buffer exchange of protein solutions
Very easy to use. However, some process optimization is needed and one needs to optimize centrifugation conditions to get consistent results.
Review Date: 15 Nov 2021 | Merck
Great product for concentrating to small volumes.
Nucleic acid concentration
The filters work great. Although you have to make sure about the amount of nucleic acid you are adding, it gets clogged and doesn't concentrate the nucleic acid efficiently if the sample is beyond the limits.
Review Date: 18 Jun 2021 | Merck
Simple product that is a massive time saver when cloning steps require recovery.
Concentration of recovered DNA fragments
These devices can save days of re-making DNA fragments when the concentration of a recovered fragment is initially too low to use in a subsequent reaction. Neat units that fit into a standard microfuge and can shrink down 4-500ul of DNA solution (or protein or other solutions) reliably into 20-25ul volume with very little loss of DNA (smaller pieces like primers do have a reduced yield). Half an hour with one of these spin columns gives around a 15-25-fold concentration of the fragment, can produce optimal fragment concentration, and allow you to skip onto the next step of the process. Simple but extremely useful.
Review Date: 17 May 2021 | Merck
Fast, efficient spinning devices that deliver consistent results!
Sample preparation
These centrifugal devices are originally meant to be used for concentrating and/or desalting macromolecules [and they work very well, yielding high, consistent recoveries]. I, however, have been using them for several "off-label" passing applications and found them invaluable! Some examples: separating polymers from extended-release pharmaceutical formulations of small-molecule type drugs prior to instrumental analysis [a great alternative to SPE], measuring in vitro-release of encapsulated drugs in liposomal formulations [a faster and more representative alternative to SPE] , separating shorter length degradation products generated during stability studies of macromolecules. They are available in a wide range of volumes and nominal MW cut-offs.
Review Date: 8 Feb 2021 | Merck
Great results.
Isolate small metabolites from biofluids
Great results that are repeatable. Good service from Merck.
Review Date: 9 Jun 2020 | Merck
Very useful and efficient but quite costly.
Purification of growth factors
Amicon Centrifugal Filter devices from Millipore are very efficient and reliable
Review Date: 20 Nov 2017 | Merck
Proteomics
This is a very reliable product from Amicon. It is very helpful in dealing with complex samples and is very easy to use. It has a reasonable pricing and fast purification.
Review Date: 12 Oct 2015 | Merck
Protein purification
This product worked well in efficiently purifying my protein. I highly recommend it.
Review Date: 26 Feb 2015 | Merck
Protein Purification
Amicon Ultra concentrator filter is really fast and amazing. As they have, even for smaller volumes, reduced the loss of protein in the sample just by sticking it on to its wall. Also they are designed in such a way that it’s very easy to use. It looks like an Eppendorf tube! I will recommend this for others for using it.
Review Date: 24 Oct 2014 | Merck
• Concentrates 500 µL down to 15 µL
• Concentration factor 25 x to 30 x
• High sample recovery (greater than 90% of dilute starting solution)
• Fast processing time - 10 to 30 minutes
• Consistent recoveries thanks to the reverse spin capability
• Reverse spin capability provides consistent recoveries
