PromoCell offers an air-liquid interface culture system to help you generate a reproducible and physiologically relevant 3D airway model using primary human bronchial epithelial cells (HBEpCs) and functional epithelial barriers.
The Air-Liquid Interface Medium is an optimized culture medium for HBEpCs. The culture medium does not contain serum or bovine pituitary extract and is designed to help you establish a physiologically relevant 3D air-liquid interface model containing HBEpC and a functional epithelial barrier.
Promocell's Air-Liquid Interface Medium contains low levels of growth factors, ensuring the survival of non-proliferating HBEpCs for up to 28 days. HBEpCs are grown on a semipermeable porous membrane that mimics the respiratory basement membrane, facilitating reproducible respiratory research. Culturing HBEpCs in the Air-Liquid Interface Medium ensures the long-term functionality of airway epithelial cells, which form an epithelial barrier containing tight junctions.
The Air-Liquid Interface Medium can also be used to induce the differentiation of Human Nasal Epithelial Cells (HNEpCs) and Human Tracheal Epithelial Cells (HTEpCs).
Moreover, the Air-Liquid Interface Medium can be used to culture 3D organoids, a mixture of apical-out or inward-oriented cilia with visible beating cilia. These functional 3D airway organoids are excellent in vitro models for high-throughput drug screening and large-scale virus-host interaction studies.