Cancer Cell Line Medium XF
Xeno-free medium for the standardized in vitro cultivation of established cancer cell lines.
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The PromoCell Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability.
Components:
The PromoCell Cancer Cell Line Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium
Note: Culture vessels need to be coated. For coating we recommend using Human Fibronectin Solution (C-43060) or Vitronectin (C-69201).
Key features:
- Suitable for long-term routine culture of adherently growing established human cancer cell lines
- Compatible with most commonly used human cancer cell lines
- Xeno-free and serum-free formulation
Standardized culture of cancer cell lines under serum and xeno-free condition
Traditional cancer cell culture methods often use fetal calf serum (FCS), which introduces variability and impacts research reliability. This application note describes the switch of an existing cancer cell culture to standardized Cancer Cell Line Medium XF - which offers a consistent, cost-effective, and xenofree environment - and subsequent routine culture.
Working with cancer stem cells
Cancer stem cells (CSCs) have an immense impact on cancer initiation, progression, metastasis, and relapse. Their ability to resist conventional therapies and drive tumor aggressiveness underscores the importance of understanding and targeting CSCs in the fight against cancer.
Download this comprehensive eBook: "Working with cancer stem cells", a complete guide on CSC biology, including an isolation protocol and the latest insights.
Cancer organoid model
In this application note, PromoCell describes a method for easy 3D organoid culture of Primary Human Dermal Fibroblasts (NHDF) and MCF- 7 cells in the Cancer Cell Line Medium XF.
Generation of human airway organoids from primary cells
In this application note, PromoCell describes the generation of functional 3D airway organoids with apical-out or inward-oriented cilia formation based on Primary Human Bronchial Epithelial Cells (HBEpC) as an in vitro model for high-throughput drug screening or large-scale virus-host interaction studies.
Relevance of HLA to cancer immunotherapy
To defend the body against threats, evolution developed the highly complex immune system with hundreds of molecular players and various cell types. This scientific poster provides an overview of the basics, as well as translational knowledge, of cancer immunotherapy.
This poster will cover:
- The genetic structure of the human leukocyte antigen complex on chromosome 6
- The distinction between major histocompatibility complex (MHC) class I and class II proteins
- An in-depth illustration of T cell activation by antigen-presenting cells (APCs)
- Application of that knowledge in cancer immunotherapy
Cancer Cell Culture: Diversity of Cells in the Tumor Micro Environment
Tumor growth is sustained by a highly diverse microenvironment, the TME. Its complex cellular interactions need serious consideration during Cancer Research.
Trending in Cancer Research: Automated Cell Identification, Unique Culture Solutions, Nanobody Development and More
Explore the techniques and technologies that are shaping the latest advancements in cancer research
Here’s Why You Should be Using Xeno-Free and Serum-Free Media for Your Cell Lines
Dr. Hagen Wieland offers interesting insight into his work developing unique culture solutions for cell-based assays, revealing how and why animal-based culture media can perturb genetic, epigenetic, and transcriptomic readouts
