VersaMAP™ Development System
The VersaMAP Development System offers our widest selection of potential analytes for Luminex-based multiplexing. The diluent supplied is suitable for most cell culture supernate samples. Samples with complex matrices, such as serum and plasma, may need further optimization. For multiplex kits validated by R&D Systems for use in complex matrices, please see our Fluorokine® MAP products. VersaMAP Development System product data…
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The VersaMAP Development System offers our widest selection of potential analytes for Luminex-based multiplexing. The diluent supplied is suitable for most cell culture supernate samples. Samples with complex matrices, such as serum and plasma, may need further optimization. For multiplex kits validated by R&D Systems for use in complex matrices, please see our Fluorokine® MAP products.
VersaMAP Development System product datasheet (PDF)
Please use the VersaMAP Development System Configuration & Ordering Tool to select up to 40 analytes to multiplex.
Features
• User defines up to 40 analytes of interest
• Wide selection of analytes
• Requires a small sample volume (<50 µL)
• Mass-calibrated standards for consistent results with every new lot of material
• Optimized antibody pairings and concentrations saves development time
• Development protocols provide directions for optimization of non-validated sample types
• Enough reagents for two 96-well plates
• Quick turn-around time: only 3-5 days
*Please allow additional time when placing orders through a distributor.
Kit Contents
• Premixed antibody-coated bead cocktail
• Premixed biotinylated detection antibody cocktail
• Standard Cocktail(s)
• Bead Diluent
• Biotin Antibody Diluent
• Standard/Sample Diluent
• Wash Buffer
• Streptavidin-PE
• 2 Filter-bottom 96-well Microplates
• Foil Plate Sealers
• Mixing Bottle
• Certificate of Analysis
A Homogeneous Multiplex Immunoassay for RTK Phosphorylation State that is Adaptable to HTS
This application note looks at the VersaMAP™ Magnetic Human RTK Multiplex Kits which are Luminex ® bead-based multiplex immunoassays for the analysis of RTK phosphorylation. These assays can be readily adapted for high-throughput screening with minimal manual intervention from cell treatment to data collection. To demonstrate this, several different cell lines were grown in 96-well plates, treated with various stimulants or inhibitors, and lysed in the same plate.
Multiplex Measurement of Biomarkers in Human Breast Cancer Cell Culture Supernates
In this application note, R&D Systems describe how the VersaMAP™ Development System can be used to rapidly screen for human biomarkers in supernates from breast cancer cell lines. The system offers an efficient method to assess cytokine profiles at a lower cost per result compared to conventional ELISAs.
