TwistAmp® Liquid Basic

Our laboratory purchased five TwistDx RPA Liquid Basic kits. Unfortunately, we were unable to obtain the expected amplification using the product, including with the positive control supplied with the kit. We undertook extensive troubleshooting. We tested multiple bags of reagent, tried different dNTPs that performed successfully in our PCR assays, and performed additional purification steps recommended by TwistDx technical support. Despite these efforts, we did not obtain the expected positive-control result. The product-performance issue itself was frustrating, but our greatest concern was the subsequent technical support and investigation process. Technical support was slow, and several recommendations were either repetitive, inconsistent with previous suggestions, or largely repeated information already available in the product documentation. Considerable laboratory time and additional reagents were consumed while attempting to resolve the problem. We eventually asked TwistDx to test the relevant product/batch themselves. Although the RPA reaction itself takes less than 30 minutes, obtaining a conclusion from the investigation required repeated follow-ups over an extended period. The case was subsequently escalated through different teams and review stages. Most concerning from a scientific perspective was the lack of transparency regarding the experimental evidence supporting the investigation. We specifically requested experimental results, including a DNA gel image, so that we could independently assess the conclusion. The final information provided to us consisted primarily of written conclusions, and the requested gel image was not provided. TwistDx ultimately stated that its investigation had not identified a product deficiency. Given that conclusion, I believe providing the underlying experimental evidence would have been particularly important. The resolution was also disappointing. We had purchased five kits and had explicitly discussed replacement of the five kits or a refund. Although we were initially informed that a replacement had been approved, we later learned that only two lyophilized kits would be provided for troubleshooting rather than replacement of the complete order. Some front-line Abbott personnel were courteous and helpful in following up, and I appreciate their efforts. However, based on our overall experience, I would rate the technical support, transparency of the quality investigation, turnaround time, and complaint resolution very poorly. For researchers considering this product, I strongly recommend taking the availability and responsiveness of post-purchase technical support into account in addition to the assay itself.

Review Date: 23 Aug 2026 | TwistDx

Easy to use and provides quality results with good after sales service.

Review Date: 18 Apr 2018 | TwistDx

It's a revolution for DNA-based analysis. It is indeed easy to use with no bulky equipment required. Of course yes, I would recommend it to my colleague.

Review Date: 18 Apr 2018 | TwistDx

Cost-effective, robust, system for developing assays which can be reliably used in research and diagnosis of infectious diseases, such as ecological field investigations with limited infrastructure or point-of-care tests.

Review Date: 18 Apr 2018 | TwistDx

This liquid system allows for higher control of TwistAmp formulations, allowing for more user control. It's very helpful in making TwistAmp products more optimizable.

Review Date: 18 Apr 2018 | TwistDx

Love it! Its a great product, saves time and money! I cannot imagine my lab without it.

Review Date: 18 Apr 2018 | TwistDx

All PCR reactions have to be re-optimized for these kits. But after that, the speed and the ease of use is amazing.

Review Date: 18 Apr 2018 | TwistDx

Cost-effective, robust, system for developing assays which can be reliably used in research and diagnosis of infectious diseases, such as ecological field investigations with limited infrastructure or point-of-care tests.

Review Date: 18 Apr 2018 | TwistDx

For POC NAT testing, This product provides all materials required for NAT testing. It is very easy to use and no expensive thermocycler is needed.

Review Date: 18 Apr 2018 | TwistDx

High-quality product, easy to use and good service.

Review Date: 18 Apr 2018 | TwistDx