Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide)
Pierce Sulfo-NHS is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.
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Review Date: 20 May 2022 | Thermo Fisher Scientific
Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2). Derivatives are easily synthesized by mixing the Sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.
Features:
- Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS
- Amine-reactive NHS esters or Sulfo-NHS esters can be made with any carboxyl-containing molecule
- Sulfo-NHS derivatives are usually directly water-soluble
- Greater than 95% by quantitative NMR; average lot purity is greater than 99%
